Irving 2015 J Clin Endocrinol Metab
| Irving BA, Lanza IR, Henderson GC, Rao RR, Spiegelman BM, Sreekumaran Nair K (2015) Combined training enhances skeletal muscle mitochondrial oxidative capacity independent of age. J Clin Endocrinol Metab [Epub ahead of print]. |
Irving BA, Lanza IR, Henderson GC, Rao RR, Spiegelman BM, Sreekumaran Nair K (2015) J Clin Endocrinol Metab
Abstract: Skeletal muscle from sedentary older adults exhibits reduced mitochondrial abundance and oxidative capacity (OXPHOS).
The primary objective was to determine whether eight weeks of combined training (CT)has more robust effect than, endurance training (ET) or resistance training (RT) on mitochondrial physiology in healthy young (18β30 y) and older (οΏ½65 y) adults.
Thirty-four young and 31 older adults were randomized to eight weeks of ET, RT, control/CT. Control subjects completed eight weeks of no exercise (control) followed by eight weeks of CT. Body composition, skeletal muscle strength, and peak oxygen uptake were measured before and after the intervention. Vastus lateralis muscle biopsies were obtained before and 48 h after the intervention. Mitochondrial physiology was evaluated by high-resolution respirometry, and expression of mitochondrial proteins and transcription factors by quantitative PCR and immunoblotting.
ET and CT significantly increased oxidative capacity and expression of mitochondrial proteins and transcription factors. All training modalities improved body composition, cardiorespiratory fitness, and skeletal muscle strength. CT induced the most robust improvements in mitochondrial related outcomes and physical characteristics despite lower training volumes for the ET and RT components. Importantly, most of the adaptations to training occurred independent of age. Conclusion: Collectively, these results demonstrate that both ET and CT increase muscle mitochondrial abundance and capacity. Although CT induced the most robust improvements in the outcomes measured. In conclusion, CT provides a robust exercise regimen to improve muscle mitochondrial outcomes and physical characteristics independent of age.
Labels: MiParea: mtDNA;mt-genetics, Exercise physiology;nutrition;life style
Organism: Human
Tissue;cell: Skeletal muscle
Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
Labels, [Epub ahead of print]
