Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Inorganic phosphate"

From Bioblast
ย 
(2 intermediate revisions by 2 users not shown)
Line 1: Line 1:
{{MitoPedia
{{MitoPedia
|abbr=P<sub>i</sub>
|abbr=P<sub>i</sub>
|description='''Inorgnic phosphate''' (Pi) is a salt of phosphoric acid. In solution near physiological pH, the species HPO<sub>4</sub><sup>2-</sup> and H<sub>2</sub>PO<sub>4</sub><sup>-</sup> dominate. ย 
|description='''Inorgnic phosphate''' (P<sub>i</sub>) is a salt of phosphoric acid. In solution near physiological pH, the species HPO<sub>4</sub><sup>2-</sup> and H<sub>2</sub>PO<sub>4</sub><sup>-</sup> dominate. ย 
''See also'': [[Phosphate carrier]] (Pic).ย  ย 
''See also'': [[Phosphate carrier]] (Pic).ย  ย 
|info=[[Scheibye-Knudsen 2009 Eur J Appl Physiol]]
|info=[[Scheibye-Knudsen 2009 Eur J Appl Physiol]]
}}
}}
__TOC__
== Application in HRR ==
:::: The P<sub>i</sub> concentration in mitochondrial respiration media should be saturating for measurement of OXPHOS capacity (10 mM in [[MiR05-Kit]]) with [[HRR]]. Many media used in mitochondrial respirometric studies contain inorganic phosphate at lower concentraitons (2 mM), which may not be saturating respiration in all pathway control states. It is not easy to isolate mitochondria in buffers without phosphate to study [[LEAK respiration]] respiration limited by the absence of phosphate.
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
|mitopedia topic=Substrate and metabolite
}}
}}
== Application in HRR ==
:::: The P<sub>i</sub> concentration in mitochondrial respiration media should be saturating for measurement of OXPHOS capacity (10 mM in [[MiR05-Kit]]) with [[HRR]]. Many media used in mitochondrial respirometric studies contain inorganic phosphate at lower concentraitons (2 mM), which may not be saturating respiration in all pathway control states. It is not easy to isolate mitochondria in buffers without phosphate to study [[LEAK]] respiration limited by the absence of phosphate.

Latest revision as of 11:18, 25 November 2020


high-resolution terminology - matching measurements at high-resolution


Inorganic phosphate

Description

Inorgnic phosphate (Pi) is a salt of phosphoric acid. In solution near physiological pH, the species HPO42- and H2PO4- dominate. See also: Phosphate carrier (Pic).

Abbreviation: Pi

Reference: Scheibye-Knudsen 2009 Eur J Appl Physiol

Application in HRR

The Pi concentration in mitochondrial respiration media should be saturating for measurement of OXPHOS capacity (10 mM in MiR05-Kit) with HRR. Many media used in mitochondrial respirometric studies contain inorganic phosphate at lower concentraitons (2 mM), which may not be saturating respiration in all pathway control states. It is not easy to isolate mitochondria in buffers without phosphate to study LEAK respiration respiration limited by the absence of phosphate.


MitoPedia topics: Substrate and metabolite