Difference between revisions of "Inorganic phosphate"

Revision as of 08:22, 26 September 2018

high-resolution terminology - matching measurements at high-resolution

Inorganic phosphate

Description

Inorgnic phosphate (Pi) is a salt of phosphoric acid. In solution near physiological pH, the species HPO₄^2- and H₂PO₄^- dominate. See also: Phosphate carrier (Pic).

Abbreviation: P_i

Reference: Scheibye-Knudsen 2009 Eur J Appl Physiol

MitoPedia topics: Substrate and metabolite

Application in HRFR

The P_i concentration in mitochondrial respiration media should be saturating for measurement of OXPHOS capacity (10 mM in MiR05-Kit) with HRFR. Many media used in mitochondrial respirometric studies contain inorganic phosphate at lower concentraitons (2 mM), which may not be saturating respiration in all pathway control states. It is not easy to isolate mitochondria in buffers without phosphate to study LEAK respiration limited by the absence of phosphate.

@@ Line 1: / Line 1: @@
 {{MitoPedia
-|abbr=Pi
+|abbr=P<sub>i</sub>
-|description=The '''inorgnic phosphate (Pi)''' concentration in mitochondrial respiration media should be saturating for measurement of OXPHOS capacity (10 mM in [[MiR06]]).
+|description='''Inorgnic phosphate''' (Pi) is a salt of phosphoric acid. In solution near physiological pH, the species HPO<sub>4</sub><sup>2-</sup> and H<sub>2</sub>PO<sub>4</sub><sup>-</sup> dominate.
-|info=[[MiPNet14.13 Medium-MiR06]]
+''See also'': [[Phosphate carrier]] (Pic).
-|type=Substrate PS
+|info=[[Scheibye-Knudsen 2009 Eur J Appl Physiol]]
-}}
-{{MitoPedia concepts|type=Substrate PS
-}}
-{{MitoPedia methods|type=Substrate PS
-}}
-{{MitoPedia O2k and high-resolution respirometry|type=Substrate PS
 }}
 {{MitoPedia topics
 |mitopedia topic=Substrate and metabolite
-|type=Substrate PS
 }}
+== Application in HRFR ==
+:::: The P<sub>i</sub> concentration in mitochondrial respiration media should be saturating for measurement of OXPHOS capacity (10 mM in [[MiR05-Kit]]) with [[HRFR]]. Many media used in mitochondrial respirometric studies contain inorganic phosphate at lower concentraitons (2 mM), which may not be saturating respiration in all pathway control states. It is not easy to isolate mitochondria in buffers without phosphate to study [[LEAK]] respiration limited by the absence of phosphate.