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| [[File:O2k-TIP2k.jpg|right|300px|caption]] | | #REDIRECT [[MiPNet16.03 IOC65]] |
| '''O2k-Workshop on High-Resolution Respirometry: O2k-Basic and TPP-Basic'''
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| Schröcken, Vorarlberg, Austria 10 - 15 December, 2011.
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| [http://www.oroboros.at/index.php?ioc-schroecken Hotel Körbersee]
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| '''Registration form''':http://www.oroboros.at/index.php?id=ioc-registration
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| For some pictures, see [[IOC61_Photos|Workshop photos]]
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| == Introductory Workshop in Parallel Sessions: O2k Basic and TPP Basic ==
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| The workshop is run in parallel sessions:''' O2k Basic''' and '''TPP Basic'''. Specific emphasis is placed on '''hands-on''' practical sessions with the instrument and the software [[DatLab 4.3]], to introduce the participants into the technical and operational details of the method. The workshop programme is complemented by combined lectures (general topics; [[Substrate-uncoupler-inhibitor titration|substrate-uncoupler-inhibitor titration SUIT protocols]]) and experiments run in "Special interest groups". Short presentations of [http://www.oroboros.at/index.php?id=ioc-abstracts 'Hot topics in Mitochondrial Physiology'] provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects. Please register for O2k Basic or TPP Basic according to your specific needs and interest.
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| * '''O2k Basic''' includes a basic introduction to the [http://www.oroboros.at/index.php?oxygraph_2k OROBOROS Oxygraph-2k], overview presentations on [http://www.oroboros.at/index.php?id=high-resolution-respirometry high-resolution respirometry (HRR)], and applications in mitochondrial physiology.
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| * '''TPP Basic''' is customized particularly for users interested in [http://www.oroboros.at/index.php?id=mipnetanalyzer MultiSensor] applications, particularly ion selective electrodes for measurement of [http://www.oroboros.at/index.php?id=membrane-potential mitochondrial membrane potential (TPP+, TPMP+)]. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) will constitue a major part. According to interest of the participant other MultiSensor applications, like [[Calcium electrode|measurement of Ca2+]], calibration and calculation of free Ca2+ in buffered solutions and [[PH electrode|acidification rate (pH)]] can be discussed/demonstrated. Please indicate your topics of interest (Ca2+, pH etc.) in advance.
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| [http://www.oroboros.at/index.php?id=nextcourse Please register] for '''O2k Basic''' or '''TPP Basic''' according to your specific needs and interest.
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| == Preliminary Programme ==
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| ::'''A: Introductory lectures and hands-on experiments for basic HRR in small working teams:'''
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| '''A1. General [[HRR]] topics'''
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| * O2k-assembly
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| * [[Polarographic oxygen sensor]] (OroboPOS) service
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| * POS calibration
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| * [[DatLab 4.3|DatLab]] software: principles and operation
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| * [[Titration-Injection-microPump]] - TIP2k.
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| '''A2. Hands-on experiments with the O2k'''
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| * Measurement of [[instrumental background oxygen flux]], manual and TIP2k-feedback control
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| * Intact cells: Respirometric reference assay with yeast, [[phosphorylation control protocol]]
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| * Preparation of [[permeabilized muscle fibre]]s
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| * HRR with permeabilized muscle fibres.
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| '''A3. Presentation and interpretation of [[oxygen flux]]'''
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| * Normalization of respiration per tissue or per [[mitochondrial marker]]
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| * Coupling control ratios
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| * Substrate control ratios
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| * Generalized flux control ratios.
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| ::'''B: Introductory lectures and hands-on experiments for basic TPP<sup>+</sup> (mitochondrial membrane potential) in small working teams'''
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| * Hands-on MultiSensor TPP<sup>+</sup> applications, see [[TPP special interest group during an International Oxygraph Course]]
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| * From TPP<sup>+</sup> to mitochondrial membrane potential: unspecific binding, partitioning, chemical side effects.
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| ::'''C: General topics'''
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| * Overview on the O2k and DatLab: High-resolution respirometry (HRR)
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| * Overview on O2k-MultiSensor applications
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| :* Mitochondrial membrane potential (TPP+ electrode)
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| :* Acidification (pH electrode), pH-stat feedback control
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| :* Nitric oxide (amperometric)
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| * Introduction to fundamental elements of mitochondrial respiratory physiology
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| * Protocols in HRR: Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
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| * Quality control - towards [[quality assurance]]
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| ::'''D: Special interest groups'''
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| * Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
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| * ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
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| * Advanced experimental protocols
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| == Participants and areas of interest ==
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| * [[Oliveira MF|'''Oliveira MF, BR''']]: Studies on the mitochondrial metabolism in the blood flow ''Schistosoma mansoni''.
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| * [[Pinho JPC|'''Pinho Joao Paulo, BR''']]: Studies on the dynamic changes of mitochondrial function following a blood meal in the kissing bug ''Rhodnius prolixus''.
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| * [[Goncalves RL|'''Goncalves Renata de Lima Sales, BR''']]: Mitochondrial membrane potential, metabolic states.
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| * [[Lau G|'''Lau Gigi, CA''']]: Physiological adaptations of mitochondria to hypoxia in fishes.
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| * [[Mc Donald A|'''Mc Donald Allison, CA''']]: Functional and regulatory characterization of alternative oxidases in various kingdoms of life.
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| * [[Keipert S|'''Keipert S, DE''']]: Mitochondrial uncoupling in skeletal muscle: Transgenic mice with ectopic expression of UCP1 in skeletal muscle with beneficial effects on energy metabolism, diet-induced obesity and aging.
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| * [[Ost M|'''Ost Mario , DE''']]: Mitochondrial uncoupling in skeletal muscle: Transgenic mice with ectopic expression of UCP1 in skeletal muscle with beneficial effects on energy metabolism, diet-induced obesity and aging.
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| * [[Gonzalez FA|'''Gonzalez Franquesa Alba, ES''']]: Mitochondrial dysfunction in insulin-dependent tissues such as liver, skeletal muscle and adipose tissue.
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| * [[Bechara Hirvonen N|'''Bechara Hirvonen Nada, FI''']]: Mitochondrial dysfunction in experimental heart failure.
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| * [[Cannon M|'''Cannon Megan, NL''']]: Metabolic remodeling in cardiac hypertrophy and heart failure.
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| == Abstract Submission ==
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| Abstracts are welcome for short presentations (hot topics in mitochondrial physiology), providing participants for an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects. Abstracts should be '''submitted by November 21, 2011''' (1 page, single spaced, including title, authors, affiliations, text and 3 references including titles).
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| *[http://wiki.oroboros.at/index.php/Special:AbstractsExtension?event=IOC65 Add your Abstract >>]
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| *[[IOC_Abstracts_MiPNet16.03|Look at submitted Abstracts >>]]
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| == Tutors ==
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| * [[Gnaiger E|'''Erich Gnaiger''']] (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
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| * [[Fasching M|'''Mario Fasching''']] (OROBOROS INSTRUMENTS, AT)
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| * [[Pesta D|'''Dominik Pesta''']] (Innsbruck Medical University, AT)
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| * [[Sumbalova Z|'''Zuzana Sumbalova''']] (OROBOROS INSTRUMENTS, AT)
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| * [[Wiethuechter A|'''Anita Wiethuechter''']] (OROBOROS INSTRUMENTS, AT)
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| [[Category:O2k-Workshops]]
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| [[Category:Events]]
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