Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Hoppel 2021 Cells"

From Bioblast
Line 8: Line 8:
|keywords=Field study, Mitochondrial function, Physical exercise, Platelets, Quality control, Respirometry
|keywords=Field study, Mitochondrial function, Physical exercise, Platelets, Quality control, Respirometry
|editor=[[Plangger M]]
|editor=[[Plangger M]]
|mipnetlab=DE Duesseldorf Roden M, AT Innsbruck Gnaiger E, AT Innsbruck Oroboros, IT Verona Calabria E
}}
}}
{{Labeling
{{Labeling

Revision as of 17:11, 17 September 2021

Publications in the MiPMap
Hoppel F, Garcia-Souza LF, Kantner-Rumplmair W, Burtscher M, Gnaiger E, Pesta D, Calabria E (2021) Human platelet mitochondrial function reflects systemic mitochondrial alterations: a protocol for application in field studies. Cells 10:2088.

ยป PMID: 34440857 Open Access

Hoppel Florian, Garcia-Souza Luiz Felipe, Kantner-Rumplmair Wilhelm, Burtscher Martin, Gnaiger Erich, Pesta Dominik, Calabria Elisa (2021) Cells

Abstract: Human blood cells may offer a minimally invasive strategy to study systemic alterations of mitochondrial function. Here we tested the reliability of a protocol designed to study mitochondrial respiratory control in human platelets (PLTs) in field studies, using high-resolution respirometry (HRR). Several factors may trigger PLT aggregation during the assay, altering the homogeneity of the cell suspension and distorting the number of cells added to the two chambers (A, B) of the Oroboros Oxygraph-2k (O2k). Thus, inter-chamber variability (โˆ†ab) was calculated by normalizing oxygen consumption to chamber volume (JO2) or to a specific respiratory control state (flux control ratio, FCR) as a reliable parameter of experimental quality. The method's reliability was tested by comparing the โˆ†ab of laboratory-performed experiments (LAB, N = 9) to those of an ultramarathon field study (three sampling time-points: before competition (PRE, N = 7), immediately after (POST, N = 10) and 24 h after competition (REC; N = 10)). Our results show that โˆ†ab JO2 changed PRE-POST, but also for LAB-POST and LAB-REC, while all โˆ†ab FCR remained unchanged. Thus, we conclude that our method is reliable for assessing PLT mitochondrial function in LAB and field studies and after systemic stress conditions. โ€ข Keywords: Field study, Mitochondrial function, Physical exercise, Platelets, Quality control, Respirometry โ€ข Bioblast editor: Plangger M โ€ข O2k-Network Lab: DE Duesseldorf Roden M, AT Innsbruck Gnaiger E, AT Innsbruck Oroboros, IT Verona Calabria E


Labels: MiParea: Respiration, Instruments;methods 


Organism: Human  Tissue;cell: Platelet  Preparation: Permeabilized cells, Intact cells 


Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k 

2021-09