Difference between revisions of "High-resolution respirometry"
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Revision as of 14:21, 21 November 2011
- high-resolution terminology - matching measurements at high-resolution
High-resolution respirometry
Description
High-resolution respirometry (HRR) is based on the OROBOROS Oxygraph-2k, combining chamber design, application of oxygen-tight materials, electrochemical sensors and electronics, Peltier-temperature control and software features (DatLab) to obtain a unique level of quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation (TIP2k). Standardized two-point calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background oxygen flux (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR.
Abbreviation: HRR
Reference: MiPNet06.01; Gnaiger_2008_POS; Gnaiger_2001_RespPhysiol
MitoPedia methods:
Respirometry,
Fluorometry,
Spectrophotometry
