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Difference between revisions of "Harrison 2015 J Appl Physiol"

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{{Publication
{{Publication
|title=Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol (1985) Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. [Epub ahead of print].
|title=Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. https://doi.org/10.1152/japplphysiol.00146.2015
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26251509 PMID: 26251509]
|info=J Appl Physiol 119:1210-8. [http://www.ncbi.nlm.nih.gov/pubmed/26251509 PMID: 26251509 Open Access]
|authors=Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E
|authors=Harrison David K, Fasching M, Fontana-Ayoub M, Gnaiger Erich
|year=2015
|year=2015
|journal=J Appl Physiol
|journal=J Appl Physiol
|abstract=Mitochondrial control of cellular redox states is a fundamental component of cell signaling in the coordination of core energy metabolism and homeostasis during normoxia and hypoxia. We investigated the relationship between cytochrome redox states and mitochondrial oxygen consumption at steady-state levels of hypoxia in mitochondria isolated from beef and mouse heart (BHImt, MHImt), comparing two species with different cardiac dynamics and local oxygen demands. A low-noise, rapid spectrophotometric system using visible light for the measurement of cytochrome redox states was combined with high-resolution respirometry. Monophasic hyperbolic relations were observed between oxygen consumption, JO2, and oxygen partial pressure, pO2, within the range <1.1 kPa (8.3 mmHg; 13 µM). p50j (pO2 at 0.5∙Jmax) was 0.015±0.0004 and 0.021±0.003 kPa (0.11 and 0.16 mmHg) for BHImt and MHImt, respectively. Maximum oxygen consumption, Jmax, was measured at saturating ADP levels (OXPHOS capacity) with Complex I-linked substrate supply. Redox states of cytochromes aa3 and c were biphasic hyperbolic functions of pO2. The relation between cytochrome oxidation state and oxygen consumption revealed a separation of distinct phases from mild to severe and deep hypoxia. When cytochrome c oxidation increased from fully reduced to 45% oxidised at 0.1 Jmax, pO2 was as low as 0.002 kPa (0.02 µM), and trace amounts of oxygen are sufficient to partially oxidize the cytochromes. At higher pO2 under severe hypoxia, respiration increases steeply while redox changes are small. Under mild hypoxia, the steep slope of oxidation of cytochrome c when flux remains more stable represents a cushioning mechanism maintaining respiration high at the onset of hypoxia.
|abstract=Mitochondrial control of cellular redox states is a fundamental component of cell signaling in the coordination of core energy metabolism and homeostasis during normoxia and hypoxia. We investigated the relationship between cytochrome redox states and mitochondrial oxygen consumption at steady-state levels of hypoxia in mitochondria isolated from beef and mouse heart (BHimt, MHimt), comparing two species with different cardiac dynamics and local oxygen demands. A low-noise, rapid spectrophotometric system using visible light for the measurement of cytochrome redox states was combined with high-resolution respirometry. Monophasic hyperbolic relations were observed between oxygen consumption, ''J''<sub>O<sub>2</sub></sub>, and oxygen partial pressure, ''p''<sub>O<sub>2</sub></sub>, within the range <1.1 kPa (8.3 mmHg; 13 µM). ''p''<sub>50''j''</sub> (''p''<sub>O<sub>2</sub></sub> at 0.5∙''J''<sub>max</sub>) was 0.015±0.0004 and 0.021±0.003 kPa (0.11 and 0.16 mmHg) for BHimt and MHimt, respectively. Maximum oxygen consumption, ''J''<sub>max</sub>, was measured at saturating ADP levels (OXPHOS capacity) with Complex I-linked substrate supply. Redox states of cytochromes ''aa''<sub>3</sub> and ''c'' were biphasic hyperbolic functions of ''p''<sub>O<sub>2</sub></sub>. The relation between cytochrome oxidation state and oxygen consumption revealed a separation of distinct phases from mild to severe and deep hypoxia. When cytochrome ''c'' oxidation increased from fully reduced to 45 % oxidised at 0.1 ''J''<sub>max</sub>, ''p''<sub>O<sub>2</sub></sub> was as low as 0.002 kPa (0.02 µM), and trace amounts of oxygen are sufficient to partially oxidize the cytochromes. At higher ''p''<sub>O<sub>2</sub></sub> under severe hypoxia, respiration increases steeply while redox changes are small. Under mild hypoxia, the steep slope of oxidation of cytochrome ''c'' when flux remains more stable represents a cushioning mechanism maintaining respiration high at the onset of hypoxia.
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS
|editor=[[Gnaiger E]]
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Oroboros
}}
}}
::::* This publication presents results obtained with a test prototype instrument of the [[O2k-Innovation description|O2k-Innovation]].
::::» More details: [[O2k-Innovation]]
[[Image:Logo MitoFit.jpg|right|120px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]
== MitoFit news 2015#14 ==
::::* 2015-09-02: Defining mt-hypoxia at cytochrome redox steady-states, with the [[O2k-Innovation description|O2k-Innovation]]. » [[K-Regio_MitoFit#2015 |MitoFit news]]
== Cited by ==
::* 8 articles in PubMed (2022-05-30) https://pubmed.ncbi.nlm.nih.gov/26251509/
{{Labeling
{{Labeling
|area=Respiration, Instruments;methods, Comparative MiP;environmental MiP
|area=Respiration, Instruments;methods, Comparative MiP;environmental MiP
|injuries=Hypoxia
|organism=Mouse, Bovines
|organism=Mouse, Bovines
|tissues=Heart
|tissues=Heart
|preparations=Isolated mitochondria
|preparations=Isolated mitochondria
|topics=O2, Oxygen
|topics=Oxygen kinetics, Redox state
|couplingstates=OXPHOS
|couplingstates=OXPHOS
|substratestates=CI
|pathways=N
|instruments=Oxygraph-2k, O2k-Spectrophotometer, Protocol
|instruments=Oxygraph-2k, TIP2k, O2k-Spectrophotometer
|additional=Epub ahead of print
|additional=MitoFitPublication, Steady state, Tissue normoxia,
MitoFit2022Hypoxia, MitoFit2022rTCA
}}
}}
* This publication presents results obtained with a test prototype instrument of the [[NextGen-O2k]].
* More details: [[NextGen-O2k innovation]]

Revision as of 07:55, 28 June 2022

Publications in the MiPMap
Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. https://doi.org/10.1152/japplphysiol.00146.2015

» J Appl Physiol 119:1210-8. PMID: 26251509 Open Access

Harrison David K, Fasching M, Fontana-Ayoub M, Gnaiger Erich (2015) J Appl Physiol

Abstract: Mitochondrial control of cellular redox states is a fundamental component of cell signaling in the coordination of core energy metabolism and homeostasis during normoxia and hypoxia. We investigated the relationship between cytochrome redox states and mitochondrial oxygen consumption at steady-state levels of hypoxia in mitochondria isolated from beef and mouse heart (BHimt, MHimt), comparing two species with different cardiac dynamics and local oxygen demands. A low-noise, rapid spectrophotometric system using visible light for the measurement of cytochrome redox states was combined with high-resolution respirometry. Monophasic hyperbolic relations were observed between oxygen consumption, JO2, and oxygen partial pressure, pO2, within the range <1.1 kPa (8.3 mmHg; 13 µM). p50j (pO2 at 0.5∙Jmax) was 0.015±0.0004 and 0.021±0.003 kPa (0.11 and 0.16 mmHg) for BHimt and MHimt, respectively. Maximum oxygen consumption, Jmax, was measured at saturating ADP levels (OXPHOS capacity) with Complex I-linked substrate supply. Redox states of cytochromes aa3 and c were biphasic hyperbolic functions of pO2. The relation between cytochrome oxidation state and oxygen consumption revealed a separation of distinct phases from mild to severe and deep hypoxia. When cytochrome c oxidation increased from fully reduced to 45 % oxidised at 0.1 Jmax, pO2 was as low as 0.002 kPa (0.02 µM), and trace amounts of oxygen are sufficient to partially oxidize the cytochromes. At higher pO2 under severe hypoxia, respiration increases steeply while redox changes are small. Under mild hypoxia, the steep slope of oxidation of cytochrome c when flux remains more stable represents a cushioning mechanism maintaining respiration high at the onset of hypoxia.

Bioblast editor: Gnaiger E O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck Oroboros

  • This publication presents results obtained with a test prototype instrument of the O2k-Innovation.
» More details: O2k-Innovation
MitoFit

MitoFit news 2015#14

Cited by


Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP 

Stress:Hypoxia  Organism: Mouse, Bovines  Tissue;cell: Heart  Preparation: Isolated mitochondria 

Regulation: Oxygen kinetics, Redox state  Coupling state: OXPHOS  Pathway:HRR: Oxygraph-2k, TIP2k, O2k-Spectrophotometer 

MitoFitPublication, Steady state, Tissue normoxia, MitoFit2022Hypoxia, MitoFit2022rTCA