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Difference between revisions of "Harrison 2015 J Appl Physiol"

From Bioblast
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|tissues=Heart
|tissues=Heart
|preparations=Isolated mitochondria
|preparations=Isolated mitochondria
|topics=O2, Oxygen
|topics=Oxygen kinetics
|couplingstates=OXPHOS
|couplingstates=OXPHOS
|substratestates=CI
|substratestates=CI

Revision as of 13:44, 1 December 2015

Publications in the MiPMap
Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. [Epub ahead of print].

ยป PMID: 26251509

Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) J Appl Physiol

Abstract: Mitochondrial control of cellular redox states is a fundamental component of cell signaling in the coordination of core energy metabolism and homeostasis during normoxia and hypoxia. We investigated the relationship between cytochrome redox states and mitochondrial oxygen consumption at steady-state levels of hypoxia in mitochondria isolated from beef and mouse heart (BHImt, MHImt), comparing two species with different cardiac dynamics and local oxygen demands. A low-noise, rapid spectrophotometric system using visible light for the measurement of cytochrome redox states was combined with high-resolution respirometry. Monophasic hyperbolic relations were observed between oxygen consumption, JO2, and oxygen partial pressure, pO2, within the range <1.1 kPa (8.3 mmHg; 13 ยตM). p50j (pO2 at 0.5โˆ™Jmax) was 0.015ยฑ0.0004 and 0.021ยฑ0.003 kPa (0.11 and 0.16 mmHg) for BHImt and MHImt, respectively. Maximum oxygen consumption, Jmax, was measured at saturating ADP levels (OXPHOS capacity) with Complex I-linked substrate supply. Redox states of cytochromes aa3 and c were biphasic hyperbolic functions of pO2. The relation between cytochrome oxidation state and oxygen consumption revealed a separation of distinct phases from mild to severe and deep hypoxia. When cytochrome c oxidation increased from fully reduced to 45% oxidised at 0.1 Jmax, pO2 was as low as 0.002 kPa (0.02 ยตM), and trace amounts of oxygen are sufficient to partially oxidize the cytochromes. At higher pO2 under severe hypoxia, respiration increases steeply while redox changes are small. Under mild hypoxia, the steep slope of oxidation of cytochrome c when flux remains more stable represents a cushioning mechanism maintaining respiration high at the onset of hypoxia.


โ€ข O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS


Labels: MiParea: Respiration, Instruments;methods, Comparative MiP;environmental MiP 


Organism: Mouse, Bovines  Tissue;cell: Heart  Preparation: Isolated mitochondria 

Regulation: Oxygen kinetics  Coupling state: OXPHOS 

HRR: Oxygraph-2k, TIP2k, O2k-Spectrophotometer, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Epub ahead of print