Edit Publication: Gnaiger 1989 Soc Exp Biol Seminar Series

You do not have permission to edit this page, for the following reason:

The action you have requested is limited to users in the group: Users.

Publication

Do you need help?	Press CTRL+left mouse click - this will open the detailed guidelines in a separate window
Reference:
Link:
Author(s):
Year:
Journal:
Abstract:	Cellular energy transformations are accompanied by heat changes indicative of physiological and metabolic activities of living organisms. The thermodynamic approach by metabolic (direct) calorimetry reveals the integrated sum of all enthalpy changes occurring within the experimental chamber, that is the living open system and its environment. The term microcalorimetry is loosely defined, indicating that heat flux is measured in the range of 1 to 1000 µW, equivalent to 0.0022 to 2.2 nmol O<sub>2</sub> s<sup>-1</sup> or 8 to 8000 µmol h<sup>-1</sup> for aerobic metabolism. Due to recent technological advancements, metabolic microcalorimetry has become a sensitive, non-invasive method for the study of respiration and complex metabolic process in animals. The classic method of indirect calorimetry depends on the calculation of heat changes from measured oxygen consumption and theoretical oxycaloric equivalents. This indirect method is extended by including anaerobic metabolite changes in the calculation of theoretical heat changes. Comparison of direct and indirect calorimetry constitutes the thermodynamic energy balance method, the crucial test for a complete biochemical description of net processes under various metabolic states. Thermochemical interpretation of biochemical and calorimetric data is not only required in energy balance studies, it is important for understanding the functional significance of heat flux in physiological energetics. Specifically, [[calorespirometry]] - the simultaneous measurement of heat flux and oxygen flux in an open flow or perfusion system - enables the partitioning of total heat flux into aerobic and anaerobic components. * Dedicated to [[Johansen K \|Kjell Johansen]]
Keyword(s):
Editor:
O2k-Network Lab(s):

Please provide some more detailed information about you publication, by ticking the appropriate keywords

MiPareas:	Respiration Instruments;methods mt-Biogenesis;mt-density mt-Structure;fission;fusion mt-Membrane mtDNA;mt-genetics nDNA;cell genetics Genetic knockout;overexpression Comparative MiP;environmental MiP Gender Developmental biology Exercise physiology;nutrition;life style mt-Medicine Patients Pharmacology;toxicology mt-Awareness
Pathological states:	Aging;senescence Alzheimer's Autism Cancer Cardiovascular COPD Diabetes Inherited Infectious Myopathy Neurodegenerative Obesity Parkinson's Sepsis Other
Stress conditions:	Cell death Cryopreservation Ischemia-reperfusion Permeability transition Oxidative stress;RONS Temperature Hypoxia Mitochondrial disease
Organism:	Human Pig Mouse Rat Guinea pig Bovines Horse Dog Rabbit Cat Other mammals Chicken Zebrafish Drosophila Artemia Caenorhabditis elegans Saccharomyces cerevisiae Birds Reptiles Amphibians Fishes Chelicerates Crustaceans Hexapods Myriapods Annelids Molluscs Nematodes Other invertebrates Fungi Plants Protists Eubacteria Archea
Tissues;cells:	Heart Skeletal muscle Nervous system Liver Kidney Lung;gill Islet cell;pancreas;thymus Endothelial;epithelial;mesothelial cell Blood cells Fat Genital Other cell lines CHO HEK HeLa Neuroblastoma Fibroblast HUVEC Macrophage-derived Lymphocyte Platelet Stem cells
Preparations:	Intact organism Intact organ Permeabilized cells Permeabilized tissue Homogenate Isolated mitochondria SMP Chloroplasts Enzyme Oxidase;biochemical oxidation Intact cells
Enzyme:	Adenine nucleotide translocase Complex I Complex II;succinate dehydrogenase Complex III Complex IV;cytochrome c oxidase Complex V;ATP synthase Inner mt-membrane transporter Marker enzyme Supercomplex TCA cycle and matrix dehydrogenases Uncoupling protein
Regulation; kinetics:	Aerobic glycolysis ADP ATP ATP production AMP Calcium Coupling efficiency;uncoupling Cyt c Flux control Inhibitor Ion;substrate transport mt-Membrane potential Oxygen kinetics PCr;Cr pH Phosphate Redox state Substrate Temperature Threshold;excess capacity Uncoupler Fatty acid Amino acid Q-junction effect
Coupling:	LEAK ROUTINE OXPHOS ET
Pathways:	F N S Gp DQ CIV NS Other combinations ROX
High-resolution respirometry:	Oxygraph-2k TIP2k O2k-Fluorometer pH NO TPP Ca O2k-Spectrophotometer O2k-Manual O2k-Protocol Theory NextGen-O2k
Additional labels:	(separate values by comma)

Free text: If the abstract is longer than 500 words, please enter additional information here.

Summary:

This is a minor edit

Watch this page

Cancel

Edit Publication: Gnaiger 1989 Soc Exp Biol Seminar Series

Navigation menu

Search