Difference between revisions of "Garedew 2005 Eur Shock Soc"
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{{Publication | {{Publication | ||
|title=Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS | |title=Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11. | ||
|info=[[Media:Garedew 2005 Eur Shock Soc.pdf |'''Bioblast Access''']] | |info=[[Media:Garedew 2005 Eur Shock Soc.pdf |'''Bioblast Access''']] | ||
|authors=Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger E | |authors=Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger E | ||
|year=2005 | |year=2005 | ||
|journal=Medimond | |journal=Medimond | ||
|abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the OROBOROS [[Oxygraph-2k]], combining instrumental design, electronics and task-specific DatLab software. | |abstract=[[High-resolution respirometry]] with mitochondria, cells or biopsies is based on the unique features of the OROBOROS [[Oxygraph-2k|O2k]], combining instrumental design, electronics and task-specific DatLab software. | ||
Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP2k]] is applied for kinetics as a modular component of the | Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump [[TIP2k]] is applied for kinetics as a modular component of the O2k. [[DatLab]] provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information. | ||
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Jansen-Duerr P, AT Innsbruck OROBOROS | |mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck Jansen-Duerr P, AT Innsbruck OROBOROS | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
Revision as of 14:16, 16 June 2015
| Garedew A, Hütter E, Haffner B, Gradl P, Gradl L, Jansen-Dürr P, Gnaiger E (2005) High-resolution respirometry for the study of mitochondrial function in health and disease. The OROBOROS O2k. Proc 11th Congress Eur Shock Soc, Vienna, Austria (Redl H, ed) Medimond, Bologna:107-11. |
Garedew A, Huetter E, Haffner B, Gradl P, Gradl L, Jansen-Duerr P, Gnaiger E (2005) Medimond
Abstract: High-resolution respirometry with mitochondria, cells or biopsies is based on the unique features of the OROBOROS O2k, combining instrumental design, electronics and task-specific DatLab software. Various respiratory states are analyzed with <0.4 million cells or <4 mg biopsy (muscle, liver) per assay. Complex substrate/inhibitor titration protocols are used to quantify functional properties of selected components of the respiratory chain. The electronic titration-injection micropump TIP2k is applied for kinetics as a modular component of the O2k. DatLab provides on-line instrumental background corrections of oxygen flux and on-line data analysis, and tabulated results are available at the end of an experiment. This sets a new standard for biomedical and clinical studies, combining high-resolution with instant diagnostic information.
• O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck Jansen-Duerr P, AT Innsbruck OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Preparation: Intact cells
Regulation: Uncoupler Coupling state: OXPHOS
HRR: Oxygraph-2k, TIP2k
