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Difference between revisions of "Gain"

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:::: The gain of the signal of the O2 channel  oxygen sensors can be adjusted in DatLab by selecting “Oxygraph” / “O2k Control” from the menue. The gain has to be adjusted to the oxygen level in your experiment, for most applications at 37°C the pre-set gain of 1 is adequate. When the gain setting is too high for your experiment the signal will reach a constant maximum value (10 V, often displayed as 9.99 V) and the flux will therefore apparently drop to zero.  
:::: The gain of the signal of the O2 channel  oxygen sensors can be adjusted in DatLab by selecting “Oxygraph” / “O2k Control” from the menue. The gain has to be adjusted to the oxygen level in your experiment, for most applications at 37°C the pre-set gain of 1 is adequate. When the gain setting is too high for your experiment the signal will reach a constant maximum value (10 V, often displayed as 9.99 V) and the flux will therefore apparently drop to zero.  
:::: [[File:Gain setting too high.png|600px|MitoFit-QCS]]


:::: For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero calibration, see also [[Air calibration]]. If you frequently switch between gains, the easiest way is to store zereo calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.
:::: For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero calibration, see also [[Air calibration]]. If you frequently switch between gains, the easiest way is to store zereo calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.

Revision as of 13:59, 25 October 2019


high-resolution terminology - matching measurements at high-resolution


Gain

Description

The gain is an amplification factor applied to an input signal to increase the output signal.


MitoPedia O2k and high-resolution respirometry: O2k hardware, DatLab 



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Gain: O2 channel

The gain of the signal of the O2 channel oxygen sensors can be adjusted in DatLab by selecting “Oxygraph” / “O2k Control” from the menue. The gain has to be adjusted to the oxygen level in your experiment, for most applications at 37°C the pre-set gain of 1 is adequate. When the gain setting is too high for your experiment the signal will reach a constant maximum value (10 V, often displayed as 9.99 V) and the flux will therefore apparently drop to zero.
MitoFit-QCS


For each gain setting a separate calibration of the oxygen signal is required. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero calibration, see also Air calibration. If you frequently switch between gains, the easiest way is to store zereo calibrations in different DatLab files and use the "read from file" function in the calibration window. Alternatively, values can be entered manually.
Oxygen signals that have been obtained after proper calibration are independent of the gain setting. Therefore oxygen concentrations (and fluxes) that have been recorded at different gain settings can of course be used together.
See also: Oxygen signal

Gain: pX channel

Gain: NO channel