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Flux baseline correction

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Revision as of 05:03, 16 March 2016 by Gnaiger Erich (talk | contribs)


high-resolution terminology - matching measurements at high-resolution


Flux baseline correction

Description

Flux baseline correction provides the option to display the plot and all values of the flux (or flow, or flux control ratio) as the total flux, J, minus a baseline flux, J0.

JV(bc) = JV - JV0
JV = (dc/dt) · ν-1 · SF - V

For the oxygen channel, JV is O2 flux per volume [pmol/(s·ml)] (or volume-specific O2 flux), c is the oxygen conentration [nmol/ml = µmol/l = µM], dc/dt is the (positive) slope of oxygen concentration over time [nmol/(s · ml)], ν-1 = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), SF=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and V is the volume-specific background oxygen flux (background correction.

Abbreviation: bc


MitoPedia O2k and high-resolution respirometry: DatLab