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Difference between revisions of "Flux baseline correction"

From Bioblast
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  ''J<sub>V</sub>''(bc) = ''J<sub>V</sub>'' - ''J<sub>V</sub>''<sub>0</sub>
  ''J<sub>V</sub>''(bc) = ''J<sub>V</sub>'' - ''J<sub>V</sub>''<sub>0</sub>
  ''J<sub>V</sub>'' = (d''c''/d''t'') · ''ν''<sup>-1</sup> · ''SF'' - ''J°<sub>V</sub>''
  ''J<sub>V</sub>'' = (d''c''/d''t'') · ''ν''<sup>-1</sup> · ''SF'' - ''J°<sub>V</sub>''
For the oxygen channel, ''J<sub>V</sub>'' is O2 flux per volume [pmol/(s·ml)] (or volume-specific O<sub>2</sub> flux), ''c'' is the oxygen conentration [nmol/ml = µmol/l = µM], d''c''/d''t'' is the (positive) slope of oxygen concentration over time [nmol/(s · ml)], ''ν''<sup>-1</sup> = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), ''SF''=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and ''J°<sub>V</sub>'' is the volume-specific background oxygen flux ([[background correction]]). ''Further details'': [[Flux / Slope]]
For the oxygen channel, ''J<sub>V</sub>'' is O2 flux per volume [pmol/(s·ml)] (or volume-specific O<sub>2</sub> flux), ''c'' is the oxygen conentration [nmol/ml = µmol/l = µM], d''c''/d''t'' is the (positive) slope of oxygen concentration over time [nmol/(s · ml)], ''ν''<sup>-1</sup> = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), ''SF''=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and ''J°<sub>V</sub>'' is the volume-specific background oxygen flux ([[background correction]]). ''Further details'': [[Flux / Slope]].
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{{MitoPedia methods
|mitopedia method=Respirometry, Fluorometry
|mitopedia method=Respirometry, Fluorometry
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|mitopedia O2k and high-resolution respirometry=DatLab
|mitopedia O2k and high-resolution respirometry=DatLab
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Revision as of 07:38, 18 March 2016


high-resolution terminology - matching measurements at high-resolution


Flux baseline correction

Description

Flux baseline correction provides the option to display the plot and all values of the flux (or flow, or flux control ratio) as the total flux, J, minus a baseline flux, J0.

JV(bc) = JV - JV0
JV = (dc/dt) · ν-1 · SF - V

For the oxygen channel, JV is O2 flux per volume [pmol/(s·ml)] (or volume-specific O2 flux), c is the oxygen conentration [nmol/ml = µmol/l = µM], dc/dt is the (positive) slope of oxygen concentration over time [nmol/(s · ml)], ν-1 = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), SF=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and V is the volume-specific background oxygen flux (background correction). Further details: Flux / Slope.

Abbreviation: bc


MitoPedia methods: Respirometry, Fluorometry 


MitoPedia O2k and high-resolution respirometry: DatLab