Fisher-Wellman 2018 Cell Rep
|Fisher-Wellman KH, Davidson MT, Narowski TM, Lin CT, Koves TR, Muoio DM (2018) Mitochondrial diagnostics: A multiplexed assay platform for comprehensive assessment of mitochondrial energy fluxes. Cell Rep 24:3593-606.|
Abstract: Chronic metabolic diseases have been linked to molecular signatures of mitochondrial dysfunction. Nonetheless, molecular remodeling of the transcriptome, proteome, and/or metabolome does not necessarily translate to functional consequences that confer physiologic phenotypes. The work here aims to bridge the gap between molecular and functional phenomics by developing and validating a multiplexed assay platform for comprehensive assessment of mitochondrial energy transduction. The diagnostic power of the platform stems from a modified version of the creatine kinase energetic clamp technique, performed in parallel with multiplexed analyses of dehydrogenase activities and ATP synthesis rates. Together, these assays provide diagnostic coverage of the mitochondrial network at a level approaching that gained by molecular "-omics" technologies. Application of the platform to a comparison of skeletal muscle versus heart mitochondria reveals mechanistic insights into tissue-specific distinctions in energy transfer efficiency. This platform opens exciting opportunities to unravel the connection between mitochondrial bioenergetics and human disease.
• Keywords: Bioenergetics, Creatine kinase clamp, Diagnostics, Heart, Methods, Mitochondria, Skeletal muscle • Bioblast editor: Plangger M • O2k-Network Lab: US NC Greenville Neufer PD, US NC Durham Koves TR
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse Tissue;cell: Heart, Skeletal muscle Preparation: Isolated mitochondria
Regulation: mt-Membrane potential, PCr;Cr Coupling state: LEAK, OXPHOS Pathway: F, N, S HRR: Oxygraph-2k, TPP