Difference between revisions of "Fatty acid oxidation"
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|description='''Fatty acid oxidation''' (β-oxidation) is a multi-step process by which [[fatty acid]]s are broken down to generate acetyl-CoA, NADH and FADH<sub>2</sub> for further energy transformation. | |description='''Fatty acid oxidation''' (β-oxidation) is a multi-step process by which [[fatty acid]]s are broken down to generate acetyl-CoA, NADH and FADH<sub>2</sub> for further energy transformation in the mitochondrial matrix. Whereas NADH is the substrate of CI, FADH<sub>2</sub> is the substrate of [[Electron-transferring flavoprotein complex]] (CETF) which is localized on the matrix face of the mtIM, and supplies electrons from FADH<sub>2</sub> to CoQ2. Before the mitochondrial transport for ß-oxidation, fatty acids (short-chain with 1-6, medium-chain with 7–12, long-chain with >12 carbon atoms) are activated by fatty acyl-CoA synthases (thiokinases) in the cytosol. For the mitochondrial transport of long-chain fatty acids the mtOM-enzyme [[carnitine palmitoyltransferase I]] (CPT-1; rate-limiting step in FAO) is required which generates an acyl-carnitine intermediate from acyl-CoA and carnitine. In the next step, an integral mtIM protein [[carnitine-acylcarnitine translocase]] (CACT) catalyzes the entrance of acyl-carnitines into the mitochondrial matrix in exchange for free carnitines. In the inner side of the mtIM, another enzyme [[carnitine palmitoyltransferase 2]] (CPT-2) converts the acyl-carnitines to carnitine and acyl-CoAs, which undergo ß-oxidation in the mitochondrial matrix. Short- and medium-chain fatty acids do not require the carnitine shuttle for mitochondrial transport. [[Octanoate]], but not [[palmitate]], (eight- and 16-carbon saturated fatty acids) may pass the mt-membranes, but both are frequently supplied to mt-preparations in the activated form of [[octanoylcarnitine]] or [[palmitoylcarnitine]]. | ||
|info=[[Gnaiger 2019 MitoPathways]] | |info=[[Gnaiger 2019 MitoPathways]], | ||
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Revision as of 17:04, 18 November 2020
- high-resolution terminology - matching measurements at high-resolution
Fatty acid oxidation
Description
Fatty acid oxidation (β-oxidation) is a multi-step process by which fatty acids are broken down to generate acetyl-CoA, NADH and FADH2 for further energy transformation in the mitochondrial matrix. Whereas NADH is the substrate of CI, FADH2 is the substrate of Electron-transferring flavoprotein complex (CETF) which is localized on the matrix face of the mtIM, and supplies electrons from FADH2 to CoQ2. Before the mitochondrial transport for ß-oxidation, fatty acids (short-chain with 1-6, medium-chain with 7–12, long-chain with >12 carbon atoms) are activated by fatty acyl-CoA synthases (thiokinases) in the cytosol. For the mitochondrial transport of long-chain fatty acids the mtOM-enzyme carnitine palmitoyltransferase I (CPT-1; rate-limiting step in FAO) is required which generates an acyl-carnitine intermediate from acyl-CoA and carnitine. In the next step, an integral mtIM protein carnitine-acylcarnitine translocase (CACT) catalyzes the entrance of acyl-carnitines into the mitochondrial matrix in exchange for free carnitines. In the inner side of the mtIM, another enzyme carnitine palmitoyltransferase 2 (CPT-2) converts the acyl-carnitines to carnitine and acyl-CoAs, which undergo ß-oxidation in the mitochondrial matrix. Short- and medium-chain fatty acids do not require the carnitine shuttle for mitochondrial transport. Octanoate, but not palmitate, (eight- and 16-carbon saturated fatty acids) may pass the mt-membranes, but both are frequently supplied to mt-preparations in the activated form of octanoylcarnitine or palmitoylcarnitine.
Abbreviation: FAO
Reference: Gnaiger 2019 MitoPathways,
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Talk:Fatty acid oxidation
FAO and HRR
- FAO cannot proceed without a substrate combination of fatty acids & malate, and inhibition of CI blocks FAO completely. Fatty acids are split stepwise into two carbon fragments forming acetyl-CoA, which enters the TCA cycle by condensation with oxaloacetate (CS reaction). Therefore, FAO implies simultaneous electron transfer into the Q-junction through CETF and CI.
- Studies with FAO in mt-preparations are conducted with mitochondrial respiration media (MiR05Cr, MiR06, etc.) with fatty acid-free Bovine serum albumine [1], [2], [3].
SUITbrowser question: Fatty acid oxidation
- SUIT protocols can assess the respiration stimulated by fatty acid oxidation, with the participation of the electron-transferring flavoprotein complex.
- The SUITbrowser can be used to find the best SUIT protocols to answer this and other research questions.
References
- ↑ Lemieux H, Semsroth S, Antretter H, Höfer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43:1729–38. »Bioblast Access«
- ↑ Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301:R1078–87. »Open Access«
- ↑ Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access«
- ↑ Oliveira AF, Cunha DA, Ladriere L, Igoillo-Esteve M, Bugliani M, Marchetti P, Cnop M (2015) In vitro use of free fatty acids bound to albumin: A comparison of protocols. Biotechniques 58:228-33. »Open Access«
- » O2k-Network discussion forum: fatty acids used in permeabilized fibre assays
- » F-pathway control state
MitoPedia methods:
Respirometry
MitoPedia topics:
Substrate and metabolite
