Difference between revisions of "FN"

Revision as of 14:25, 22 January 2019

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FN

Description

FN is induced in mt-preparations by addition of NADH-generating substrates (N-pathway control state, or CI-linked pathway control) in combination with one or several fatty acids, which are supplied to feed electrons into the F-junction through fatty acyl CoA dehydrogenase (reduced form FADH2), to electron transferring flavoprotein (CETF), and further through the Q-junction to Complex III (CIII). FAO not only depends on electron transfer through the F-junction (which is typically rate-limiting), but simultaneously generates FADH₂ and NADH and thus depends on N-junction throughput. Hence FAO can be inhibited completely by inhibition of Complex I (CI). This physiological substrate combination is required for partial reconstitution of TCA cycle function and convergent electron-input into the Q-junction, to compensate for metabolite depletion into the incubation medium. FS in combination exerts an additive effect of convergent electron flow in most types of mitochondria.

Abbreviation: FN

Reference: Electron transfer-pathway state, Gnaiger 2014 MitoPathways

MitoPedia concepts: Respiratory state, SUIT state, Recommended

MitoPedia topics: EAGLE

Communicated by Gnaiger E, edited 2019-01-22 by Komlodi T.

» O2k-Network discussion forum: fatty acids used in permeabilized fibre assays

» F-junction

» Fatty acid oxidation

» N-junction

» F(N)

FN_L

FN pathway in the LEAK state can be evaluated in the following SUIT protocols:

FN_P

FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:

SUIT-002

DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-002 O2 mt D005
DL-Protocol for permeabilized fibers (pfi): SUIT-002 O2 pfi D006
DL-Protocol for permeabilized cells (pce): SUIT-002 O2 pce D007

SUIT-005

DL-Protocol for permeabilized fibers (pfi):SUIT-005 O2 pfi D011

FN_E

FN pathway in the ET state can be evaluated in the following SUIT protocols:

SUIT-019

@@ Line 16: / Line 16: @@
 ::::» [[Fatty acid oxidation]]
 ::::» [[N-junction]]
-::::» [[F(N)]]
+::::» [[Fatty acid oxidation pathway control state|F(N)]]
-== FN(L) ==
+== FN<sub>''L''</sub> ==
+FN pathway in the LEAK state can be evaluated in the following SUIT protocols:
+== FN<sub>''P''</sub> ==
+FN pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
+:::*[[SUIT-002]]
+::::* DL-Protocol for isolated mitochondria and tissue homogenate (mt): [[SUIT-002 O2 mt D005]]
+::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-002 O2 pfi D006]]
+::::* DL-Protocol for permeabilized cells (pce): [[SUIT-002 O2 pce D007]]
+:::*[[SUIT-005]]
+::::* DL-Protocol for permeabilized fibers (pfi):[[SUIT-005 O2 pfi D011]]
+:::*[[SUIT-015]]
+:::*[[SUIT-016]]
+:::*[[SUIT-017]]
-== FN(P) ==
+== FN<sub>''E''</sub>==
-:::[[SUIT-002]]
+FN pathway in the ET state can be evaluated in the following SUIT protocols:
-:::[[SUIT-002 O2 mt D005]]
+:::*[[SUIT-019]]
-:::[[SUIT-002 O2 pfi D006]]
-:::[[SUIT-002 O2 pce D007]]
-:::[[SUIT-005]]
-:::[[SUIT-005 O2 pfi D011]]
-:::[[SUIT-015]]
-:::[[SUIT-016]]
-:::[[SUIT-017]]
-== FN(E) ==
-:::[[SUIT-019]]