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FCCP

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FCCP

Description

Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C10H5F3N4O); uncoupler, F(x): FCCP added at concentration x.

Abbreviation: Fx

Reference: MiPNet10.04; SteinlechnerMaran_1996_AJP


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Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 


Application in HRR

F: FCCP (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C10H5F3N4O); Sigma C 2920, 10 mg, store at 4 °C: FW = 254.17)

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 1.27 mg of FCCP directly into a 5 ml glass vial.
2) add 5 ml Ethanol
3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
4) store in glass vials (recommendation) at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 1 µl steps using a 10 µl syringe
  • Final conc. in 2 ml O2k-chamber: 0.5 µM steps