Difference between revisions of "FCCP"
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}} | }} | ||
{{Labeling | {{Labeling | ||
|kinetics=Inhibitor; Uncoupler | |kinetics=Inhibitor; Uncoupler | ||
|topics=Respiration; OXPHOS; ETS Capacity | |topics=Respiration; OXPHOS; ETS Capacity | ||
|instruments=Chemicals; Media | |||
|type=Uncoupler | |||
}} | }} | ||
== Application in [[HRR]] == | |||
'''F: FCCP''' (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C<sub>10</sub>H<sub>5</sub>F<sub>3</sub>N<sub>4</sub>O); Sigma C 2920, 10 mg, store at 4 °C: FW = 254.17) | |||
<span style="color:#8B008B"> '''Caution:''' Very toxic. Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.</span> | |||
'''Preparation of 4 mg/ml stock solution''' (dissolved in H<sub>2</sub>0): | |||
::1) weigh 4 mg of Malonic acid directly into a 2 ml glass vial | |||
::2) add 1 ml H<sub>2</sub>0 | |||
::3) divide into 0.2 ml portions | |||
::4) store frozen at -20 °C | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | |||
::* Titration volume: 1 µl steps using a 10 µl syringe | |||
::* Final conc. in 2 ml O2k-chamber: 0.5 µM steps | |||
Revision as of 14:28, 22 November 2010
- high-resolution terminology - matching measurements at high-resolution
FCCP
Description
Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C10H5F3N4O); uncoupler, F(x): FCCP added at concentration x.
Abbreviation: Fx
Reference: MiPNet10.04; SteinlechnerMaran_1996_AJP
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
F: FCCP (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C10H5F3N4O); Sigma C 2920, 10 mg, store at 4 °C: FW = 254.17)
Caution: Very toxic. Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.
Preparation of 4 mg/ml stock solution (dissolved in H20):
- 1) weigh 4 mg of Malonic acid directly into a 2 ml glass vial
- 2) add 1 ml H20
- 3) divide into 0.2 ml portions
- 4) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl steps using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 µM steps
