Difference between revisions of "FCCP"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=U<sub>''c''</sub> | ||
|description='''FCCP''' (Carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone) is a protonophore or [[uncoupler]] | |description='''FCCP''' (Carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone) is a protonophore or [[uncoupler]]: '''FCCP''' added at uncoupler concentration U<sub>''c''</sub>; ''c'' is the [[optimum uncoupler concentration]] in titrations to obtain maximum mitochondrial respiration in the [[noncoupled respiration|noncoupled]] state of [[ETS capacity]]. | ||
|info=[[MiPNet10.04]]; [[SteinlechnerMaran_1996_AJP]] | |info=[[MiPNet10.04]]; [[SteinlechnerMaran_1996_AJP]] | ||
|type=Uncoupler | |type=Uncoupler | ||
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'''Preparation of 1 mM stock solution''' (dissolved in 99.9% Ethanol): | '''Preparation of 1 mM stock solution''' (dissolved in 99.9% Ethanol): | ||
::1) | ::1) Weigh 1.27 mg of FCCP into a glass vial. | ||
::2) | ::2) Dissolve in 5 ml ethanol. | ||
::3) | ::3) Divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution. | ||
::3) | ::3) Store in glass vials (recommendation) at -20 °C. | ||
Revision as of 14:39, 30 December 2013
- high-resolution terminology - matching measurements at high-resolution
FCCP
Description
FCCP (Carbonyl cyanide p-trifluoro-methoxyphenyl hydrazone) is a protonophore or uncoupler: FCCP added at uncoupler concentration Uc; c is the optimum uncoupler concentration in titrations to obtain maximum mitochondrial respiration in the noncoupled state of ETS capacity.
Abbreviation: Uc
Reference: MiPNet10.04; SteinlechnerMaran_1996_AJP
MitoPedia topics: Uncoupler
CCCP versus FCCP
Bioblast alert 2013(02): We replace FCCP by the less expensive CCCP in our experiments with high-resultion respirometry (HRR).
Application in HRR
F: FCCP (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C10H5F3N4O); Sigma C 2920, 10 mg, store at 4 °C: FW = 254.17)
Caution: Toxic!
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):
- 1) Weigh 1.27 mg of FCCP into a glass vial.
- 2) Dissolve in 5 ml ethanol.
- 3) Divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution.
- 3) Store in glass vials (recommendation) at -20 °C.
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl steps using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 µM steps
Frequently, more diluted FCCP for getting several titration steps is necessary. In this case prepare more diluted solution (ditution 2-10x) from your stock solution.
