Difference between revisions of "FCCP"
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'''F: FCCP''' (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C<sub>10</sub>H<sub>5</sub>F<sub>3</sub>N<sub>4</sub>O); Sigma C 2920, 10 mg, store at 4 °C: FW = 254.17) | '''F: FCCP''' (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C<sub>10</sub>H<sub>5</sub>F<sub>3</sub>N<sub>4</sub>O); Sigma C 2920, 10 mg, store at 4 °C: FW = 254.17) | ||
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<span style="color:#8B008B"> '''Caution:''' Toxic!</span> | |||
<span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
Revision as of 15:02, 15 May 2013
- high-resolution terminology - matching measurements at high-resolution
FCCP
Description
FCCP (synonym: Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone) uncoupler, Fc: FCCP added at concentration c; c is the optimum uncoupler concentration in titrations of FCCP to obtain maximum mitochondrial respiration in the noncoupled state of ETS capacity.
Abbreviation: Fc
Reference: MiPNet10.04; SteinlechnerMaran_1996_AJP
MitoPedia topics: Uncoupler
Application in HRR
F: FCCP (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C10H5F3N4O); Sigma C 2920, 10 mg, store at 4 °C: FW = 254.17)
Caution: Toxic!
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 1.27 mg of FCCP into a glass vial
- 2) dissolve in 5 ml Ethanol
- 3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 3) store in glass vials (recommendation) at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl steps using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 µM steps
Frequently, more diluted FCCP for getting several titration steps is necessary. In this case prepare more diluted solution (ditution 2-10x) from your stock solution.
