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Difference between revisions of "FCCP"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=Fx
|abbr=F<sub>''c''</sub>
|description=Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C<sub>10</sub>H<sub>5</sub>F<sub>3</sub>N<sub>4</sub>O); [[uncoupler]], F(x): '''FCCP''' added at concentration x.
|description=Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C<sub>10</sub>H<sub>5</sub>F<sub>3</sub>N<sub>4</sub>O); [[uncoupler]], F<sub>''c''</sub> or F''c'': '''FCCP''' added at concentration ''c''.
|info=[[MiPNet10.04]]; [[SteinlechnerMaran_1996_AJP]]
|info=[[MiPNet10.04]]; [[SteinlechnerMaran_1996_AJP]]
|type=Uncoupler
|type=Uncoupler
}}
{{MitoPedia methods|type=Uncoupler
}}
}}
{{MitoPedia topics
{{MitoPedia topics
Line 9: Line 11:
|type=Uncoupler
|type=Uncoupler
}}
}}
== Application in [[HRR]] ==
== Application in [[HRR]] ==



Revision as of 08:36, 16 November 2011


high-resolution terminology - matching measurements at high-resolution


FCCP

Description

Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C10H5F3N4O); uncoupler, Fc or Fc: FCCP added at concentration c.

Abbreviation: Fc

Reference: MiPNet10.04; SteinlechnerMaran_1996_AJP



MitoPedia topics: Uncoupler 

Application in HRR

F: FCCP (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C10H5F3N4O); Sigma C 2920, 10 mg, store at 4 ยฐC: FW = 254.17)

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 1.27 mg of FCCP into a glass vial and dissolve in 5 ml Ethanol
2) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
3) store in glass vials (recommendation) at -20 ยฐC

Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 1 ยตl steps using a 10 ยตl syringe
  • Final conc. in 2 ml O2k-chamber: 0.5 ยตM steps

Frequently, more diluted FCCP for getting several titration steps is necessary. In this case prepare more diluted solution (ditution 2-10x) from your stock solution.