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Difference between revisions of "FCCP"

From Bioblast
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|type=Uncoupler
|type=Uncoupler
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{{Labeling
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|kinetics=Inhibitor; Uncoupler
|topics=Respiration; OXPHOS; ETS Capacity
|instruments=Chemicals; Media
|type=Uncoupler
}}
== Application in [[HRR]] ==
== Application in [[HRR]] ==



Revision as of 16:39, 9 March 2011


high-resolution terminology - matching measurements at high-resolution


FCCP

Description

Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C10H5F3N4O); uncoupler, F(x): FCCP added at concentration x.

Abbreviation: Fx

Reference: MiPNet10.04; SteinlechnerMaran_1996_AJP


MitoPedia topics: Uncoupler 


Application in HRR

F: FCCP (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C10H5F3N4O); Sigma C 2920, 10 mg, store at 4 ยฐC: FW = 254.17)

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.


Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 1.27 mg of FCCP into a glass vial and dissolve in 5 ml Ethanol
2) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
3) store in glass vials (recommendation) at -20 ยฐC

Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 1 ยตl steps using a 10 ยตl syringe
  • Final conc. in 2 ml O2k-chamber: 0.5 ยตM steps

Frequently, more diluted FCCP for getting several titration steps is necessary. In this case prepare more diluted solution (ditution 2-10x) from your stock solution.