Difference between revisions of "Extrinsic fluorophores"
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|description='''Extrinsic fluorophores''' are molecules labelled with a fluorescent dye (as opposed to intrinsic fluorescence or autofluorescence of molecules which does not require such labelling). They are available for a wide range of parameters including ROS (H<sub>2</sub>O<sub>2</sub>, [[Amplex red]]) (HOO<sup>-</sup>, MitoSOX) , mitochondrial membrane potential ([[Safranin]], JC1, TMRM, Rhodamin 123), Ca<sup>2+</sup> ([[Fura2]], Indo 1, Calcium Green) , pH (Fluorescein, HPTS, SNAFL-1), Mg<sup>2+</sup> (Magnesium orange) and redox state (roGFP). | |description='''Extrinsic fluorophores''' are molecules labelled with a fluorescent dye (as opposed to intrinsic fluorescence or autofluorescence of molecules which does not require such labelling). They are available for a wide range of parameters including ROS (H<sub>2</sub>O<sub>2</sub>, [[Amplex red]]) (HOO<sup>-</sup>, MitoSOX) , mitochondrial membrane potential ([[Safranin]], JC1, TMRM, Rhodamin 123), Ca<sup>2+</sup> ([[Fura2]], Indo 1, Calcium Green) , pH (Fluorescein, HPTS, SNAFL-1), Mg<sup>2+</sup> (Magnesium orange) and redox state (roGFP). | ||
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{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry | |mitopedia method=Fluorometry | ||
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Revision as of 23:58, 27 January 2016
- high-resolution terminology - matching measurements at high-resolution
Extrinsic fluorophores
Description
Extrinsic fluorophores are molecules labelled with a fluorescent dye (as opposed to intrinsic fluorescence or autofluorescence of molecules which does not require such labelling). They are available for a wide range of parameters including ROS (H2O2, Amplex red) (HOO-, MitoSOX) , mitochondrial membrane potential (Safranin, JC1, TMRM, Rhodamin 123), Ca2+ (Fura2, Indo 1, Calcium Green) , pH (Fluorescein, HPTS, SNAFL-1), Mg2+ (Magnesium orange) and redox state (roGFP).
MitoPedia methods: Fluorometry
