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E-L coupling efficiency

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high-resolution terminology - matching measurements at high-resolution


E-L coupling efficiency

Description

ETS coupling efficiency The ETS coupling efficiency (E-L coupling control factor) is a normalized flux ratio, j≈E = ≈E/E = (E-L)/E = 1-L/E. j≈E is 0.0 at zero coupling (L=E) and 1.0 at the limit of a fully coupled system (L=0). The background state is the LEAK state which is stimulated to ETS reference state by uncoupler titration. LEAK states LN or LT may be stimulated first by saturating ADP (State P) with subsequent uncoupler titration to State E. The ETS coupling efficiency is based on measurement of a coupling control ratio (LEAK control ratio, L/E), whereas the thermodynamic or ergodynamic efficiency of coupling between ATP production (DT phosphorylation) and oxygen consumption is based on measurement of the output/input flux ratio (~P/O2 ratio) and output/input force ratio (Gibbs force of phosphorylation/Gibbs force of oxidation). Biochemical coupling efficiency is either expressed as the ETS coupling efficiency, j≈E, or OXPHOS coupling efficiency, j≈P, obtained in a coupling control protocol (phosphorylation control protocol). » MiPNet article

Abbreviation: j≈E

Reference: Flux control factor


MitoPedia concepts: Respiratory control ratio 


MitoPedia methods: Respirometry 

Biochemical coupling efficiency: from 0 to <1

Publications in the MiPMap
Gnaiger E (2015) Biochemical coupling efficiency: from 0 to <1. Mitochondr Physiol Network 2015-01-18.


OROBOROS (2015) MiPNet

Abstract: Zooming in on biochemical coupling efficiency, j≈E compared to j≈P.


O2k-Network Lab: AT Innsbruck Gnaiger E


Labels:




Regulation: Coupling efficiency;uncoupling  Coupling state: LEAK, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Theory 


Quantification of coupling of mitochondrial respiration is a fundamental component of OXPHOS analysis.[1],[2] Biochemical coupling efficiency is distinguished from ergodynamic efficiency.[3],[4],[5]

Coupling control states for j≈E

» Respiratory state, ETS-competent pathway control state, Electron transfer system


Flux control ratio and flux control factor

» Flux control ratio, FCR, Flux control factor, FCF


Compare

mt-Preparations

OXPHOS coupling efficiency OXPHOS coupling efficiency, P-L control factor: j≈P = ≈P/P = (P-L)/P = 1-L/P
netOXPHOS control ratio netOXPHOS control ratio, ≈P/E control ratio: ≈P/E = (P-L)/E
OXPHOS OXPHOS capacity, P = -ROX

Intact cells

ROUTINE coupling efficiency ROUTINE coupling efficiency, (R-L or ≈R control factor): j≈R = ≈R/R = (R-L)/R = 1-L/R
netROUTINE control ratio netROUTINE control ratio, ≈R/E control ratio: ≈R/E = (R-L)/E
ROUTINE ROUTINE respiration, R = -ROX


References

  1. Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »Open Access«
  2. Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »Uncoupler«
  3. Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. »Open Access«
  4. Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109. »Bioblast Access«
  5. Gnaiger E (2015) Cell ergometry: OXPHOS and ETS coupling efficiency. Mitochondr Physiol Network 2015-01-18. »Bioblast link«


MitoPedia: related terms

Coupling control factors: biochemical efficiencies

OXPHOS coupling efficiency OXPHOS coupling efficiency, (P-L or ≈P control factor): j≈P = ≈P/P = (P-L)/P = 1-L/P
ROUTINE coupling efficiency ROUTINE coupling efficiency: j≈R = ≈R/R =(R-L)/R = 1-L/R
ETS coupling efficiency ETS coupling efficiency, E-L coupling control factor: j≈E = ≈E/E = (E-L)/E = 1-L/E

Coupling control factors: apparent excess capacity factors

Excess E-P capacity factor Excess E-P capacity factor, E-P coupling control factor: jExP = (E-P)/E = 1-P/E
Excess E-R capacity factor Excess E-R capacity factor, E-R coupling control factor: jExR = (E-R)/E = 1-R/E

Coupling control ratios

» Coupling control ratio
L/P coupling control ratio L/P coupling control ratio: L/P
L/R coupling control ratio L/R coupling control ratio, L/R
LEAK control ratio LEAK control ratio, L/E
OXPHOS control ratio OXPHOS control ratio, P/E
ROUTINE control ratio ROUTINE control ratio, R/E
netOXPHOS control ratio netOXPHOS control ratio, ≈P/E control ratio: ≈P/E = (P-L)/E
netROUTINE control ratio netROUTINE control ratio, ≈R/E control ratio: ≈R/E = (R-L)/E


List of publications: ETS and LEAK