Difference between revisions of "E-L coupling efficiency"
| Line 31: | Line 31: | ||
=== Efficiency === | === Efficiency === | ||
* [[Efficiency]] - [[Ergodynamic efficiency]] <ref> Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. [[Gnaiger 1993 Pure Appl Chem |»Open Access]]</ref>,<ref>Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109. [[Gnaiger 1993 Hypoxia |»Bioblast Access]]</ref> | * [[Efficiency]] - [[Ergodynamic efficiency]] <ref> Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. [[Gnaiger 1993 Pure Appl Chem |»Open Access]]</ref>,<ref>Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109. [[Gnaiger 1993 Hypoxia |»Bioblast Access]]</ref> | ||
* [[Biochemical coupling efficiency]] | |||
=== Coupling === | === Coupling === | ||
* Is respiration uncoupled - noncoupled - dyscoupled? <ref>Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »[[Uncoupler]]</ref> | * Is respiration uncoupled - noncoupled - dyscoupled? <ref>Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »[[Uncoupler]]</ref> | ||
* [[Coupling control factor]] | |||
[[File:EPL-free and excess.jpg|right|400px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]] | |||
=== Coupling control factors: biochemical efficiencies === | |||
:: [[Image:j--P.jpg|50 px|link=OXPHOS coupling efficiency |OXPHOS coupling efficiency]] [[OXPHOS coupling efficiency]], (''P-L'' or ''≈P'' control factor): ''j<sub>≈P</sub>'' = ''≈P/P'' = (''P-L'')/''P'' = 1-''L/P'' | |||
:: [[Image:j--R.jpg|50 px|link=ROUTINE coupling efficiency |ROUTINE coupling efficiency]] [[ROUTINE coupling efficiency]]: ''j<sub>≈R</sub>'' = ''≈R/R'' =(''R-L'')/''R'' = 1-''L/R'' | |||
:: [[Image:j--E.jpg|50 px|link=ETS coupling efficiency |ETS coupling efficiency]] [[ETS coupling efficiency]], ''E-L'' coupling control factor: ''j<sub>≈E</sub>'' = ''≈E/E'' = (''E-L'')/''E'' = 1-''L/E'' | |||
=== Coupling control factors: apparent excess capacity factors === | |||
:: [[Image:jExP.jpg|50 px|link=Excess E-P capacity factor |Excess ''E-P'' capacity factor]] [[Excess E-P capacity factor |Excess ''E-P'' capacity factor]], ''E-P'' coupling control factor: ''j<sub>ExP</sub>'' = (''E-P'')/''E'' = 1-''P/E'' | |||
:: [[Image:jExR.jpg|50 px|link=Excess E-R capacity factor |Excess ''E-R'' capacity factor]] [[Excess E-R capacity factor |Excess ''E-R'' capacity factor]], ''E-R'' coupling control factor: ''j<sub>ExR</sub>'' = (''E-R'')/''E'' = 1-''R/E'' | |||
=== Coupling control ratios === | === Coupling control ratios === | ||
:::» [[Coupling control ratio]] | :::» [[Coupling control ratio]] | ||
::[[Image:L over P.jpg|50 px|link=L/P coupling control ratio |''L/P'' coupling control ratio]] [[L/P coupling control ratio |''L/P'' coupling control ratio]]: ''L/P'' | |||
::[[Image:L over R.jpg|50 px|link=L/R coupling control ratio |''L/R'' coupling control ratio]] [[L/R coupling control ratio |''L/R'' coupling control ratio]], ''L/R'' | |||
:: [[Image:L over E.jpg|50 px|link=LEAK control ratio |LEAK control ratio]] [[LEAK control ratio]], ''L/E'' | |||
:: [[Image:P over E.jpg|50 px|link=OXPHOS control ratio |OXPHOS control ratio]] [[OXPHOS control ratio]], ''P/E'' | |||
:: [[Image:R over E.jpg|50 px|link=ROUTINE control ratio |ROUTINE control ratio]] [[ROUTINE control ratio]], ''R/E'' | |||
:: [[Image:NetP over E.jpg|60 px|link=NetOXPHOS control ratio |netOXPHOS control ratio]] [[netOXPHOS control ratio]], ''≈P/E'' control ratio: ''≈P/E'' = (''P-L'')/''E'' | |||
:: [[Image:NetR over E.jpg|60 px|link=NetROUTINE control ratio |netROUTINE control ratio]] [[netROUTINE control ratio]], ''≈R/E'' control ratio: ''≈R/E'' = (''R-L'')/''E'' | |||
| Line 53: | Line 67: | ||
=== Basics === | === Basics === | ||
* [[Electron transfer system]] | * [[Electron transfer system]] | ||
* [[Flux control ratio]] | |||
* [[Flux control factor]] | * [[Flux control factor]] | ||
* [[Coupling control protocol]] (Phosphorylation control protocol) | * [[Coupling control protocol]] (Phosphorylation control protocol) | ||
== References == | == References == | ||
Revision as of 11:00, 19 January 2015
- high-resolution terminology - matching measurements at high-resolution
E-L coupling efficiency
Description
.jpg){kind=small}
The ETS coupling efficiency (E-L coupling control factor) is a normalized flux ratio, j≈E = ≈E/E = (E-L)/E = 1-L/E. j≈E is 0.0 at zero coupling (L=E) and 1.0 at the limit of a fully coupled system (L=0). The LEAK state is stimulated to ETS by uncoupler titration. LEAK states LN or LT may be stimulated first by saturating ADP (State P) with subsequent uncoupler titration to State E. The ETS coupling efficiency is based on measurement of a coupling control ratio (LEAK control ratio, L/E), whereas the thermodynamic or ergodynamic efficiency of coupling between ATP production (DT phosphorylation) and oxygen consumption is based on measurement of the output/input flux ratio (~P/O2 ratio) and output/input force ratio (Gibbs force of phosphorylation/Gibbs force of oxidation). Biochemical coupling efficiency is either expressed as the ETS coupling efficiency, j≈E, or OXPHOS coupling efficiency, j≈P.
» MiPNet article
Abbreviation: j≈E
Reference: Flux control factor
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory control ratio"Respiratory control ratio" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Biochemical coupling efficiency: from 0 to <1
| Gnaiger E (2014) Biochemical coupling efficiency: from 0 to <1. Mitochondr Physiol Network 2014-04-18. |
Abstract: Zooming in on biochemical coupling efficiency.
• O2k-Network Lab: AT Innsbruck Gnaiger E
Labels:
Regulation: Coupling efficiency;uncoupling
Coupling state: LEAK, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Theory
Related terms in MitoPedia
Efficiency
Coupling
- Is respiration uncoupled - noncoupled - dyscoupled? [3]
- Coupling control factor
Coupling control factors: biochemical efficiencies
OXPHOS coupling efficiency, (P-L or ≈P control factor): j≈P = ≈P/P = (P-L)/P = 1-L/P
ROUTINE coupling efficiency: j≈R = ≈R/R =(R-L)/R = 1-L/R
ETS coupling efficiency, E-L coupling control factor: j≈E = ≈E/E = (E-L)/E = 1-L/E
Coupling control factors: apparent excess capacity factors
Excess E-P capacity factor, E-P coupling control factor: jExP = (E-P)/E = 1-P/E
Excess E-R capacity factor, E-R coupling control factor: jExR = (E-R)/E = 1-R/E
Coupling control ratios
L/P coupling control ratio: L/P
L/R coupling control ratio, L/R
LEAK control ratio, L/E
OXPHOS control ratio, P/E
ROUTINE control ratio, R/E
netOXPHOS control ratio, ≈P/E control ratio: ≈P/E = (P-L)/E
netROUTINE control ratio, ≈R/E control ratio: ≈R/E = (R-L)/E
Relevant respiratory states
Basics
- Electron transfer system
- Flux control ratio
- Flux control factor
- Coupling control protocol (Phosphorylation control protocol)
References
- ↑ Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. »Open Access
- ↑ Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109. »Bioblast Access
- ↑ Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »Uncoupler
- ↑ Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. »Open Access
