Difference between revisions of "E-L coupling efficiency"
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{{MitoPedia | {{MitoPedia | ||
|abbr=''j<sub>≈E</sub> | |abbr=''j<sub>≈E</sub> | ||
|description=[[Image:j--E.jpg|50 px| | |description=[[Image:j--E.jpg|50 px|ET-pathway coupling efficiency]] The '''ET-pathway coupling efficiency''' (''E-L'' coupling control factor) is a normalized flux ratio, ''j<sub>≈E</sub>'' = ''≈E/E'' = (''E-L'')/''E'' = 1-''L/E''. ''j<sub>≈E</sub>'' is 0.0 at zero coupling (''L''=''E'') and 1.0 at the limit of a fully coupled system (''L''=0). The background state is the [[LEAK]] state which is stimulated to [[ET-pathway]] reference state by [[uncoupler]] titration. LEAK states ''L''<sub>N</sub> or ''L''<sub>T</sub> may be stimulated first by saturating ADP (State ''P'') with subsequent uncoupler titration to State ''E''. The ET-pathway coupling efficiency is based on measurement of a [[coupling control ratio]] ([[LEAK control ratio]], ''L/E''), whereas the thermodynamic or [[ergodynamic efficiency]] of coupling between ATP production (DT phosphorylation) and oxygen consumption is based on measurement of the output/input flux ratio (~P/O<sub>2</sub> ratio) and output/input force ratio (Gibbs force of phosphorylation/Gibbs force of oxidation). [[Biochemical coupling efficiency]] is either expressed as the ET-pathway coupling efficiency, ''j<sub>≈E</sub>'', or [[OXPHOS coupling efficiency]], ''j<sub>≈P</sub>'', obtained in a [[coupling control protocol]] (phosphorylation control protocol). | ||
» [[#Biochemical_coupling_efficiency:_from_0_to_.3C1 | '''MiPNet article''']] | » [[#Biochemical_coupling_efficiency:_from_0_to_.3C1 | '''MiPNet article''']] | ||
|info=[[Flux control factor]] | |info=[[Flux control factor]] | ||
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{{Labeling | {{Labeling | ||
|topics=Coupling efficiency;uncoupling | |topics=Coupling efficiency;uncoupling | ||
|couplingstates=LEAK, | |couplingstates=LEAK, ET-pathway | ||
|instruments=Theory | |instruments=Theory | ||
}} | }} | ||
:::: Quantification of coupling of mitochondrial respiration is a fundamental component of OXPHOS analysis.<ref>Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. [[Gnaiger 2014 MitoPathways |»Open Access]]«</ref>,<ref>Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »[[Uncoupler]]«</ref> [[Biochemical coupling efficiency]] is distinguished from [[ergodynamic efficiency]].<ref>Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. [[Gnaiger 1993 Pure Appl Chem |»Open Access]]«</ref>,<ref>Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109. [[Gnaiger 1993 Hypoxia |»Bioblast Access]]«</ref>,<ref>Gnaiger E (2015) Cell ergometry: OXPHOS and | :::: Quantification of coupling of mitochondrial respiration is a fundamental component of OXPHOS analysis.<ref>Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. [[Gnaiger 2014 MitoPathways |»Open Access]]«</ref>,<ref>Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »[[Uncoupler]]«</ref> [[Biochemical coupling efficiency]] is distinguished from [[ergodynamic efficiency]].<ref>Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. [[Gnaiger 1993 Pure Appl Chem |»Open Access]]«</ref>,<ref>Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109. [[Gnaiger 1993 Hypoxia |»Bioblast Access]]«</ref>,<ref>Gnaiger E (2015) Cell ergometry: OXPHOS and ET-pathway coupling efficiency. Mitochondr Physiol Network 2015-01-18. [[OXPHOS_coupling_efficiency#Cell_ergometry:_OXPHOS_and_ETS_coupling_efficiency |»Bioblast link]]«</ref> | ||
[[File:EPL-free and excess.jpg|right|400px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]] | [[File:EPL-free and excess.jpg|right|400px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]] | ||
== Coupling control states for ''j<sub>≈E</sub>'' == | == Coupling control states for ''j<sub>≈E</sub>'' == | ||
::::» [[Respiratory state]], [[ETS-competent pathway control state]], [[Electron transfer system]] | ::::» [[Respiratory state]], [[ETS-competent pathway control state]], [[Electron transfer system]] | ||
::::* [[Reference state]], ''Z<sub>X</sub>'': [[Image:E.jpg|link=ETS capacity| | ::::* [[Reference state]], ''Z<sub>X</sub>'': [[Image:E.jpg|link=ETS capacity|ET-capacity]] [[ET-capacity]], ''E'' = ''E´''-ROX | ||
::::* [[Background state]], ''Y<sub>X</sub>'': [[Image:L.jpg|link=LEAK respiration|LEAK]] [[LEAK respiration]], ''L'' = ''L´''-ROX | ::::* [[Background state]], ''Y<sub>X</sub>'': [[Image:L.jpg|link=LEAK respiration|LEAK]] [[LEAK respiration]], ''L'' = ''L´''-ROX | ||
::::* [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:E-L.jpg|50 px|link=Free ETS capacity |Free ETS capacity]] [[Free | ::::* [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:E-L.jpg|50 px|link=Free ETS capacity |Free ETS capacity]] [[Free ET-capacity]], ''≈E'' = ''E-L'' | ||
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= List of publications: | = List of publications: ET-pathway and LEAK = | ||
{{#ask:[[Category:Publications]] [[Coupling states::LEAK]] [[Coupling states::ETS]] | {{#ask:[[Category:Publications]] [[Coupling states::LEAK]] [[Coupling states:: ETS]] | ||
|?Was published in year=Year | |?Was published in year=Year | ||
|?Has title=Reference | |?Has title=Reference | ||
Revision as of 16:00, 16 October 2017
- high-resolution terminology - matching measurements at high-resolution
E-L coupling efficiency
Description
.jpg){kind=small}
The ET-pathway coupling efficiency (E-L coupling control factor) is a normalized flux ratio, j≈E = ≈E/E = (E-L)/E = 1-L/E. j≈E is 0.0 at zero coupling (L=E) and 1.0 at the limit of a fully coupled system (L=0). The background state is the LEAK state which is stimulated to ET-pathway reference state by uncoupler titration. LEAK states LN or LT may be stimulated first by saturating ADP (State P) with subsequent uncoupler titration to State E. The ET-pathway coupling efficiency is based on measurement of a coupling control ratio (LEAK control ratio, L/E), whereas the thermodynamic or ergodynamic efficiency of coupling between ATP production (DT phosphorylation) and oxygen consumption is based on measurement of the output/input flux ratio (~P/O2 ratio) and output/input force ratio (Gibbs force of phosphorylation/Gibbs force of oxidation). Biochemical coupling efficiency is either expressed as the ET-pathway coupling efficiency, j≈E, or OXPHOS coupling efficiency, j≈P, obtained in a coupling control protocol (phosphorylation control protocol).
» MiPNet article
Abbreviation: j≈E
Reference: Flux control factor
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry
Biochemical coupling efficiency: from 0 to <1
| Gnaiger E (2015) Biochemical coupling efficiency: from 0 to <1. Mitochondr Physiol Network 2015-01-18. |
Abstract: Zooming in on biochemical coupling efficiency, j≈E compared to j≈P.
• O2k-Network Lab: AT Innsbruck Gnaiger E
Labels:
Regulation: Coupling efficiency;uncoupling
Coupling state: LEAK, ET-pathway"ET-pathway" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Theory
- Quantification of coupling of mitochondrial respiration is a fundamental component of OXPHOS analysis.[1],[2] Biochemical coupling efficiency is distinguished from ergodynamic efficiency.[3],[4],[5]
The Blue Book 2014: Fig. 2.4.
Coupling control states for j≈E
- » Respiratory state, ETS-competent pathway control state, Electron transfer system
- Reference state, ZX:
ET-capacity, E = E´-ROX - Background state, YX:
LEAK respiration, L = L´-ROX - Metabolic control variable, X=ZX-YX:
Free ET-capacity, ≈E = E-L
- Reference state, ZX:
- » Respiratory state, ETS-competent pathway control state, Electron transfer system
Flux control ratio and flux control factor
- » Flux control ratio, FCR, Flux control factor, FCF
- Coupling control ratio, YX/ZX:
LEAK control ratio (L/E coupling control ratio), L/E - Coupling control factor, 1-YX/ZX:
ETS coupling efficiency: j≈E = ≈E/E =(E-L)/E = 1-L/E
- Coupling control ratio, YX/ZX:
- » Flux control ratio, FCR, Flux control factor, FCF
Compare
mt-Preparations
OXPHOS coupling efficiency, P-L control factor: j≈P = ≈P/P = (P-L)/P = 1-L/P
netOXPHOS control ratio, ≈P/E control ratio: ≈P/E = (P-L)/E
OXPHOS capacity, P = P´-ROX
Intact cells
ROUTINE coupling efficiency, (R-L or ≈R control factor): j≈R = ≈R/R = (R-L)/R = 1-L/R
netROUTINE control ratio, ≈R/E control ratio: ≈R/E = (R-L)/E
ROUTINE respiration, R = R´-ROX
References
- ↑ Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »Open Access«
- ↑ Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »Uncoupler«
- ↑ Gnaiger E (1993) Nonequilibrium thermodynamics of energy transformations. Pure Appl Chem 65: 1983-2002. »Open Access«
- ↑ Gnaiger E (1993) Efficiency and power strategies under hypoxia. Is low efficiency at high glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillart G (eds) CRC Press, Boca Raton, Ann Arbor, London, Tokyo: 77-109. »Bioblast Access«
- ↑ Gnaiger E (2015) Cell ergometry: OXPHOS and ET-pathway coupling efficiency. Mitochondr Physiol Network 2015-01-18. »Bioblast link«
Coupling control factors: biochemical efficiencies
- OXPHOS coupling efficiency, (P-L or ≈P control factor): j≈P = ≈P/P = (P-L)/P = 1-L/P
- ROUTINE coupling efficiency: j≈R = ≈R/R =(R-L)/R = 1-L/R
- ETS coupling efficiency, E-L coupling control factor: j≈E = ≈E/E = (E-L)/E = 1-L/E
Coupling control factors: apparent excess capacity factors
Excess E-P capacity factor, E-P coupling control factor: jExP = (E-P)/E = 1-P/E
Excess E-R capacity factor, E-R coupling control factor: jExR = (E-R)/E = 1-R/E
Coupling control ratios
- » Coupling control ratio
L/P coupling control ratio: L/P
L/R coupling control ratio, L/R- LEAK control ratio, L/E
OXPHOS control ratio, P/E
ROUTINE control ratio, R/E- netOXPHOS control ratio, ≈P/E control ratio: ≈P/E = (P-L)/E
- netROUTINE control ratio, ≈R/E control ratio: ≈R/E = (R-L)/E
