E-L net ET capacity

high-resolution terminology - matching measurements at high-resolution

E-L net ET capacity

Description

The free ETS capacity, ≈E, is the ETS capacity corrected for LEAK respiration, ≈E = E-L. ≈E is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ETS state, therefore, is partitioned into the free ETS capacity, ≈E, and resting LEAK respiration, L_P, compensating for proton leaks, slip and cation cycling: E = ≈E+L_P (see free OXPHOS capacity).

Abbreviation: ≈E

Reference: Gnaiger 2014 MitoPathways

MitoPedia methods: Respirometry 

MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 

The Blue Book 2014: Fig. 2.4.

Coupling control states for ≈E

» Respiratory state

• Reference state, Z_X: ETS capacity, E = E´-ROX
• Background state, Y_X: LEAK respiration, L = L´-ROX
• Metabolic control variable, X=Z_X-Y_X: Scope of uncoupler stimulation, free ETS capacity, ≈E = E-L

Flux control factor

» Flux control factor, FCF

• Coupling control factor, 1-Y_X/Z_X: E-L coupling control factor, ETS coupling efficiency: j_≈E = ≈E/E =(E-L)/E = 1-L/E

Compare

• mt-Preparations Free OXPHOS capacity, ≈P = P-L
• Intact cells Free ROUTINE activity, ≈R = R-L