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Difference between revisions of "E-L net ET capacity"

From Bioblast
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[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]]
[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]]
== Coupling control states for ''≈E'' ==
::» [[Respiratory state]]
* [[Reference state]], ''Z<sub>X</sub>'': [[Image:E.jpg|link=ETS capacity|ETS capacity]] [[ETS capacity]], ''E'' = ''E´''-ROX
* [[Background state]], ''Y<sub>X</sub>'': [[Image:L.jpg|link=LEAK respiration|LEAK]] [[LEAK respiration]], ''L'' = ''L´''-ROX
* [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:E-L.jpg|50 px|link=Free ETS capacity |Free ETS capacity]] Scope of uncoupler stimulation, [[free ETS capacity]], ''≈E'' = ''E-L''
== Flux control factor ==
::» [[Flux control factor]], ''FCF''
* [[Coupling control factor]], 1-''Y<sub>X</sub>/Z<sub>X</sub>'': [[Image:j--E.jpg|50 px|link=ETS coupling efficiency |ETS coupling efficiency]] ''E-L'' coupling control factor, [[ETS coupling efficiency]]: ''j<sub>≈E</sub>'' = ''≈E/E'' =(''E-L'')/''E'' = 1-''L/E''
== Compare ==
== Compare ==
* [[Free OXPHOS capacity]]
=== mt-Preparations ===
:: [[Image:P-L.jpg|50 px|link=Free OXPHOS capacity |Free OXPHOS capacity]] [[Free OXPHOS capacity]], ''≈P'' = ''P-L''
:: [[Image:NetP over E.jpg|60 px|link=NetOXPHOS control ratio |netOXPHOS control ratio]] [[netOXPHOS control ratio]], ''≈P/E'' control ratio: ''≈P/E'' = (''P-L'')/''E''
:: [[Image:j--P.jpg|50 px|link=OXPHOS coupling efficiency |OXPHOS coupling efficiency]] [[OXPHOS coupling efficiency]], ''P-L'' control factor: ''j<sub>≈P</sub>'' = ''≈P/P'' = (''P-L'')/''P'' = 1-''L/P''
=== Intact cells ===
:: [[Image:R-L.jpg|50 px|link=Free ROUTINE activity |Free ROUTINE activity]] [[Free ROUTINE activity]], ''≈R'' = ''R-L''

Revision as of 18:12, 19 January 2015


high-resolution terminology - matching measurements at high-resolution


E-L net ET capacity

Description

Free ETS capacity The free ETS capacity, ≈E, is the ETS capacity corrected for LEAK respiration, ≈E = E-L. ≈E is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ETS state, therefore, is partitioned into the free ETS capacity, ≈E, and resting LEAK respiration, LP, compensating for proton leaks, slip and cation cycling: E = ≈E+LP (see free OXPHOS capacity).

Abbreviation: ≈E

Reference: Gnaiger 2014 MitoPathways


MitoPedia methods: Respirometry 


MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. 

Coupling control states for ≈E

» Respiratory state


Flux control factor

» Flux control factor, FCF


Compare

mt-Preparations

Free OXPHOS capacity Free OXPHOS capacity, ≈P = P-L
netOXPHOS control ratio netOXPHOS control ratio, ≈P/E control ratio: ≈P/E = (P-L)/E
OXPHOS coupling efficiency OXPHOS coupling efficiency, P-L control factor: j≈P = ≈P/P = (P-L)/P = 1-L/P

Intact cells

Free ROUTINE activity Free ROUTINE activity, ≈R = R-L