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Difference between revisions of "E-L net ET capacity"

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{{MitoPedia
{{MitoPedia
|abbr=''≈E''
|abbr=''E-L''
|description=[[Image:E-L.jpg|50 px|Free ET-capacity]] The '''Free ET-capacity''', ''≈E'', is the [[ET capacity]] corrected for [[LEAK respiration]], ''≈E'' = ''E-L''. ''≈E'' is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ET-pathway state, therefore, is partitioned into the free ET capacity, ''≈E'', and LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''E'' = ''≈E''+''L<sub>P</sub>'' (see [[free OXPHOS capacity]]).
|description=[[Image:E-L.jpg|50 px|E-L net ET capacity]] The '''''E-L'' net ET capacity''' is the [[ET capacity]] corrected for [[LEAK respiration]]. ''E-L'' is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ET-pathway state, therefore, is partitioned into the ''E-L'' net ET capacity and LEAK respiration ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''E'' = ''E-L''+''L<sub>P</sub>'' (see [[P-L net OXPHOS capacity]]).
|info=[[Gnaiger 2014 MitoPathways]]
|info=[[Gnaiger 2020 BEC MitoPathways]]
}}
}}
{{MitoPedia concepts
  Communicated by [[Gnaiger E]] (2014-09-21) last upate 2020-11-11.
|mitopedia concept=Respiratory state, Recommended
}}
{{MitoPedia topics
|mitopedia topic=EAGLE
}}
  Communicated by [[Gnaiger E]] 2014-09-21, edited 2015-01-19.
[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]]
 
== Coupling control states for ''≈E'' ==
::» [[Respiratory state]]
* [[Reference state]], ''Z<sub>X</sub>'': [[Image:E.jpg|link=ET capacity|ET capacity]] [[ET capacity]], ''E'' = ''E´''-ROX
* [[Background state]], ''Y<sub>X</sub>'': [[Image:L.jpg|link=LEAK respiration|LEAK]] [[LEAK respiration]], ''L'' = ''L´''-ROX
* [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:E-L.jpg|50 px|link=Free ET-capacity |Free ET-capacity]] Scope of uncoupler stimulation, [[Free ET-capacity]], ''≈E'' = ''E-L''
 
 
== Flux control factor ==
::» [[Flux control factor]], ''FCF''
* [[Coupling control factor]], 1-''Y<sub>X</sub>/Z<sub>X</sub>'': [[Image:j--E.jpg|50 px|link=ET-pathway coupling efficiency |ET-pathway coupling efficiency]] ''E-L'' coupling control factor, [[ET-pathway coupling efficiency]]: ''j<sub>≈E</sub>'' = ''≈E/E'' =(''E-L'')/''E'' = 1-''L/E''
 


== Compare ==
== Keywords ==
* mt-Preparations [[Image:P-L.jpg|50 px|link=Free OXPHOS capacity |Free OXPHOS capacity]] [[Free OXPHOS capacity]], ''≈P'' = ''P-L''
::::* Expand Bioblast links to '''''E-L'' net ET capacity'''
* Intact cells [[Image:R-L.jpg|50 px|link=Free ROUTINE activity |Free ROUTINE activity]] [[Free ROUTINE activity]], ''≈R'' = ''R-L''
{{Template:Keywords: Coupling control}}

Latest revision as of 00:32, 31 December 2020


high-resolution terminology - matching measurements at high-resolution


E-L net ET capacity

Description

E-L net ET capacity The E-L net ET capacity is the ET capacity corrected for LEAK respiration. E-L is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ET-pathway state, therefore, is partitioned into the E-L net ET capacity and LEAK respiration LP, compensating for proton leaks, slip and cation cycling: E = E-L+LP (see P-L net OXPHOS capacity).

Abbreviation: E-L

Reference: Gnaiger 2020 BEC MitoPathways

Communicated by Gnaiger E (2014-09-21) last upate 2020-11-11.

Keywords

  • Expand Bioblast links to E-L net ET capacity


Questions.jpg


Click to expand or collaps
Bioblast links: Coupling control - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

1. Mitochondrial and cellular respiratory rates in coupling-control states

OXPHOS-coupled energy cycles. Source: The Blue Book
» Baseline state
Respiratory rate Defining relations Icon
OXPHOS capacity P = -Rox P.jpg mt-preparations
ROUTINE respiration R = -Rox R.jpg living cells
ET capacity E = -Rox E.jpg » Level flow
» Noncoupled respiration - Uncoupler
LEAK respiration L = -Rox L.jpg » Static head
» LEAK state with ATP
» LEAK state with oligomycin
» LEAK state without adenylates
Residual oxygen consumption Rox L = -Rox ROX.jpg
  • Chance and Williams nomenclature: respiratory states
» State 1 —» State 2 —» State 3 —» State 4 —» State 5

2. Flux control ratios related to coupling in mt-preparations and living cells

» Flux control ratio
» Coupling-control ratio
» Coupling-control protocol
FCR Definition Icon
L/P coupling-control ratio L/P L/P coupling-control ratio » Respiratory acceptor control ratio, RCR = P/L
L/R coupling-control ratio L/R L/R coupling-control ratio
L/E coupling-control ratio L/E L/E coupling-control ratio » Uncoupling-control ratio, UCR = E/L (ambiguous)
P/E control ratio P/E P/E control ratio
R/E control ratio R/E R/E control ratio » Uncoupling-control ratio, UCR = E/L
net P/E control ratio (P-L)/E net P/E control ratio
net R/E control ratio (R-L)/E net R/E control ratio

3. Net, excess, and reserve capacities of respiration

Respiratory net rate Definition Icon
P-L net OXPHOS capacity P-L P-L net OXPHOS capacity
R-L net ROUTINE capacity R-L R-L net ROUTINE capacity
E-L net ET capacity E-L E-L net ET capacity
E-P excess capacity E-P E-P excess capacity
E-R reserve capacity E-R E-R reserve capacity

4. Flux control efficiencies related to coupling-control ratios

» Flux control efficiency jZ-Y
» Background state
» Reference state
» Metabolic control variable
Coupling-control efficiency Definition Icon Canonical term
P-L control efficiency jP-L = (P-L)/P = 1-L/P P-L control efficiency P-L OXPHOS-flux control efficiency
R-L control efficiency jR-L = (R-L)/R = 1-L/R R-L control efficiency R-L ROUTINE-flux control efficiency
E-L coupling efficiency jE-L = (E-L)/E = 1-L/E E-L coupling efficiency E-L ET-coupling efficiency » Biochemical coupling efficiency
E-P control efficiency jE-P = (E-P)/E = 1-P/E E-P control efficiency E-P ET-excess flux control efficiency
E-R control efficiency jE-R = (E-R)/E = 1-R/E E-R control efficiency E-R ET-reserve flux control efficiency

5. General

» Basal respiration
» Cell ergometry
» Dyscoupled respiration
» Dyscoupling
» Electron leak
» Electron-transfer-pathway state
» Hyphenation
» Oxidative phosphorylation
» Oxygen flow
» Oxygen flux
» Permeabilized cells
» Phosphorylation system
» Proton leak
» Proton slip
» Respiratory state
» Uncoupling