Difference between revisions of "E-L net ET capacity"

Revision as of 19:16, 7 November 2020

high-resolution terminology - matching measurements at high-resolution

E-L net ET capacity

Description

The free ET capacity, E-L, is the ET capacity corrected for LEAK respiration. E-L is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ET-pathway state, therefore, is partitioned into the free ET capacity, E-L, and LEAK respiration, L_P, compensating for proton leaks, slip and cation cycling: E = E-L+L_P (see free OXPHOS capacity).

Abbreviation: E-L

Reference: Gnaiger 2020 MitoPathways

Communicated by Gnaiger E (2014-09-21) last upate 2020-11-07.

The Blue Book 2020

Coupling control states for E-L

» Respiratory state

Reference state, rate Z_X: ET capacity; E = E´-Rox
Background state, rate Y_X: LEAK respiration; L = L´-Rox
Metabolic control variable, X=Z_X-Y_X: Scope of uncoupler stimulation, Free ET capacity E-L

Flux control efficiency

» Flux control efficiency, j_Z-Y

Coupling control efficiency, 1-Y_X/Z_X: E-L coupling control efficiency, ET-coupling efficiency: j_E-L = (E-L)/E = 1-L/E

Compare

mt-Preparations Free OXPHOS capacity, P-L
Intact cells Free ROUTINE activity, R-L

@@ Line 1: / Line 1: @@
 {{MitoPedia
-|abbr=''≈E''
+|abbr=''E-L''
-|description=[[Image:E-L.jpg|50 px|Free ET capacity]] The '''Free ET capacity''', ''≈E'', is the [[ET capacity]] corrected for [[LEAK respiration]], ''≈E'' = ''E-L''. ''≈E'' is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ET-pathway state, therefore, is partitioned into the free ET capacity, ''≈E'', and LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''E'' = ''≈E''+''L<sub>P</sub>'' (see [[free OXPHOS capacity]]).
+|description=[[Image:E-L.jpg|50 px|Free ET capacity]] The '''free ET capacity''', ''E-L'', is the [[ET capacity]] corrected for [[LEAK respiration]]. ''E-L'' is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ET-pathway state, therefore, is partitioned into the free ET capacity, ''E-L'', and LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''E'' = ''E-L''+''L<sub>P</sub>'' (see [[free OXPHOS capacity]]).
-|info=[[Gnaiger 2014 MitoPathways]]
+|info=[[Gnaiger 2020 MitoPathways]]
 }}
-{{MitoPedia concepts
+ Communicated by [[Gnaiger E]] (2014-09-21) last upate 2020-11-07.
-|mitopedia concept=Respiratory state, Recommended, Ergodynamics
+[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2020 MitoPathways |The Blue Book 2020]]]]
-}}
-{{MitoPedia methods}}
-{{MitoPedia O2k and high-resolution respirometry}}
-{{MitoPedia topics
-|mitopedia topic=EAGLE
-}}
-Communicated by [[Gnaiger E]] 2014-09-21, edited 2015-01-19.
-[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]]
-== Coupling control states for ''≈E'' ==
+== Coupling control states for ''E-L'' ==
 ::» [[Respiratory state]]
-* [[Reference state]], ''Z<sub>X</sub>'': [[Image:E.jpg|link=ET capacity|ET capacity]] [[ET capacity]]; ''E'' = ''E´''-''Rox''
+* [[Reference state]], rate ''Z<sub>X</sub>'': [[Image:E.jpg|link=ET capacity|ET capacity]] [[ET capacity]]; ''E'' = ''E´''-''Rox''
-* [[Background state]], ''Y<sub>X</sub>'': [[Image:L.jpg|link=LEAK respiration|LEAK]] [[LEAK respiration]]; ''L'' = ''L´''-''Rox''
+* [[Background state]], rate ''Y<sub>X</sub>'': [[Image:L.jpg|link=LEAK respiration|LEAK]] [[LEAK respiration]]; ''L'' = ''L´''-''Rox''
-* [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:E-L.jpg|50 px|link=Free ET capacity |Free ET capacity]] Scope of uncoupler stimulation, [[Free ET capacity]]; ''≈E'' = ''E-L''
+* [[Metabolic control variable]], ''X=Z<sub>X</sub>-Y<sub>X</sub>'': [[Image:E-L.jpg|50 px|link=Free ET capacity |Free ET capacity]] Scope of uncoupler stimulation, [[Free ET capacity]] ''E-L''
-== Flux control factor ==
+== Flux control efficiency ==
-::» [[Flux control factor]], ''FCF''
+::» [[Flux control efficiency]], ''j<sub>Z-Y</sub>''
-* [[Coupling control factor]], 1-''Y<sub>X</sub>/Z<sub>X</sub>'': [[Image:j--E.jpg|50 px|link=ET-coupling efficiency |ET-coupling efficiency]] ''E-L'' coupling control factor, [[ET-coupling efficiency]]: ''j<sub>≈E</sub>'' = ''≈E/E'' =(''E-L'')/''E'' = 1-''L/E''
+* [[Coupling control efficiency]], 1-''Y<sub>X</sub>/Z<sub>X</sub>'': [[Image:j--E.jpg|50 px|link=ET-coupling efficiency |ET-coupling efficiency]] ''E-L'' coupling control efficiency, [[ET-coupling efficiency]]: ''j<sub>E-L</sub>'' = (''E-L'')/''E'' = 1-''L/E''
 == Compare ==
-* mt-Preparations [[Image:P-L.jpg|50 px|link=Free OXPHOS capacity |Free OXPHOS capacity]] [[Free OXPHOS capacity]], ''≈P'' = ''P-L''
+* mt-Preparations [[Image:P-L.jpg|50 px|link=Free OXPHOS capacity |Free OXPHOS capacity]] [[Free OXPHOS capacity]], ''P-L''
-* Intact cells [[Image:R-L.jpg|50 px|link=Free ROUTINE activity |Free ROUTINE activity]] [[Free ROUTINE activity]], ''≈R'' = ''R-L''
+* Intact cells [[Image:R-L.jpg|50 px|link=Free ROUTINE activity |Free ROUTINE activity]] [[Free ROUTINE activity]], ''R-L''