E-L net ET capacity

high-resolution terminology - matching measurements at high-resolution

E-L net ET capacity

Description

The Free ET-capacity, ≈E, is the ET-capacity corrected for LEAK respiration, ≈E = E-L. ≈E is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ET-pathway state, therefore, is partitioned into the free ET-capacity, ≈E, and LEAK respiration, L_P, compensating for proton leaks, slip and cation cycling: E = ≈E+L_P (see free OXPHOS capacity).

Abbreviation: ≈E

Reference: Gnaiger 2014 MitoPathways

MitoPedia concepts: Respiratory state, Recommended 

MitoPedia topics: EAGLE 

Communicated by Gnaiger E 2014-09-21, edited 2015-01-19.

The Blue Book 2014: Fig. 2.4.

Coupling control states for ≈E

» Respiratory state

• Reference state, Z_X: ET-capacity; E = E´-Rox
• Background state, Y_X: LEAK respiration; L = L´-Rox
• Metabolic control variable, X=Z_X-Y_X: Scope of uncoupler stimulation, Free ET-capacity; ≈E = E-L

Flux control factor

» Flux control factor, FCF

• Coupling control factor, 1-Y_X/Z_X: E-L coupling control factor, ET-coupling efficiency: j_≈E = ≈E/E =(E-L)/E = 1-L/E

Compare

• mt-Preparations Free OXPHOS capacity, ≈P = P-L
• Intact cells Free ROUTINE activity, ≈R = R-L