Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Duskova 2015 Mol Microbiol

From Bioblast
Revision as of 08:20, 9 November 2016 by Beno Marija (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Publications in the MiPMap
Dušková M, Ferreira C, Lucas C, Sychrová H (2015) Two glycerol uptake systems contribute to the high osmotolerance of Zygosaccharomyces rouxii. Mol Microbiol 97:541-59.

» PMID: 25943012

Duskova M, Ferreira C, Lucas C, Sychrova H (2015) Mol Microbiol

Abstract: The accumulation of glycerol is essential for yeast viability upon hyperosmotic stress. Here we show that the osmotolerant yeast Zygosaccharomyces rouxii has two genes, ZrSTL1 and ZrSTL2, encoding transporters mediating the active uptake of glycerol in symport with protons, contributing to cell osmotolerance and intracellular pH homeostasis. The growth of mutants lacking one or both transporters is affected depending on the growth medium, carbon source, strain auxotrophies, osmotic conditions and the presence of external glycerol. These transporters are localised in the plasma membrane, they transport glycerol with similar kinetic parameters and besides their expected involvement in the cell survival of hyperosmotic stress, they surprisingly both contribute to an efficient survival of hypoosmotic shock and to the maintenance of intracellular pH homeostasis under non-stressed conditions. Unlike STL1 in S. cerevisiae, the two Z. rouxii STL genes are not repressed by glucose, but their expression and activity are downregulated by fructose and upregulated by non-fermentable carbon sources, with ZrSTL1 being more influenced than ZrSTL2. In summary, both transporters are highly important, though Z. rouxii CBS 732T cells do not use external glycerol as a source of carbon.

Labels: MiParea: Respiration, nDNA;cell genetics, Comparative MiP;environmental MiP 

Organism: Fungi 

Preparation: Intact cells 

Coupling state: ROUTINE 

HRR: Oxygraph-2k, pH