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Dlaskova 2010 Biochim Biophys Acta

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Dlasková A, Clarke KJ, Porter RK (2010) The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue. Biochim Biophys Acta 1797:1470-6.

» PMID: 20416274 Open Access; PDF

Dlaskova A, Clarke KJ, Porter RK (2010) Biochim Biophys Acta

Abstract: We provide evidence that ablation or inhibition of, uncoupling protein 1 increases the rate of reactive oxygen containing species production by mitochondria from brown adipose tissue, no matter what electron transport chain substrate is used (succinate, glycerol-3-phosphate or pyruvate/malate). Consistent with these data are our observations that (a) the mitochondrial membrane potential is maximal when uncoupling protein 1 is ablated or inhibited and (b) oxygen consumption rates in mitochondria from uncoupling protein 1 knock-out mice, are significantly lower than those from wild-type mice, but equivalent to those from wild-type mice in the presence of GDP. In summary, we show that uncoupling protein 1 can affect reactive oxygen containing species production by isolated mitochondria from brown adipose tissue. Keywords: Mitochondria, Reactive oxygen species, Brown adipose tissue, Uncoupling protein 1

O2k-Network Lab: IE Dublin Porter RK

Labels: MiParea: Respiration, Genetic knockout;overexpression 

Stress:Oxidative stress;RONS  Organism: Mouse  Tissue;cell: Fat  Preparation: Isolated mitochondria  Enzyme: Uncoupling protein 

Coupling state: OXPHOS 

HRR: Oxygraph-2k