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Difference between revisions of "Distefano 2012 Abstract IOC68"

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{{Abstract
{{Abstract
|title=Distefano G, Dube JJ, Helbling NL, Ritov VB, Stefanovic-Racic M, Toledo FGS, Ng J, Goodpaster B, Coen P (2012) Human skeletal muscle mitochondria respiration: The influence of aging, adiposity and aerobic fitness. IOC68_MiPNet17.08
|title=Distefano G, Dube JJ, Helbling NL, Ritov VB, Stefanovic-Racic M, Toledo FGS, Ng J, Goodpaster B, Coen P (2012) Human skeletal muscle mitochondria respiration: The influence of aging, adiposity and aerobic fitness. MiPNet17.08
|authors=Distefano G, Dube JJ, Helbling NL, Ritov VB, Stefanovic-Racic M, Toledo FGS, Ng J, Goodpaster B, Coen P
|authors=Distefano G, Dube JJ, Helbling NL, Ritov VB, Stefanovic-Racic M, Toledo FGS, Ng J, Goodpaster B, Coen P
|year=2012
|year=2012

Revision as of 15:00, 27 March 2012

Distefano G, Dube JJ, Helbling NL, Ritov VB, Stefanovic-Racic M, Toledo FGS, Ng J, Goodpaster B, Coen P (2012) Human skeletal muscle mitochondria respiration: The influence of aging, adiposity and aerobic fitness. MiPNet17.08

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Distefano G, Dube JJ, Helbling NL, Ritov VB, Stefanovic-Racic M, Toledo FGS, Ng J, Goodpaster B, Coen P (2012)

Event: IOC68

Background: Aging is associated with reductions in skeletal muscle mitochondria function as evidenced by a decreased capacity for ATP production and mitochondrial protein content (1,2,3). Aging is also associated with changes in body composition, including increased adiposity, and a loss of aerobic fitness. Both are factors that confound an examination of the relationship between mitochondrial function and aging per se. The objective of this study was to determine whether the respiratory properties of permeabilized skeletal muscle fibers are altered with chronological age, or more related to age associated changes in adiposity and aerobic fitness.

Methods: A total of 63 participants were assigned to one of the following groups: Young (Y, 26.9 ± 0.9 yrs, n=30), Middle-aged (M, 41.2 ± 2.4 yrs, n=13), or Elderly (77.7 ± 1.1 yrs, n=20). Following an overnight fast, a percutaneous muscle biopsy of vastus lateralis was obtained. Maximal coupled (St 3), maximal uncoupled (St U), and LEAK state (St L) respiration was determined in saponin permeabilized muscle fiber bundles using high-resolution respirometry. VO2 peak was determined by a graded exercise test. Total body fat and fat free mass were assessed by whole body DEXA.

Results: The Y group had significantly greater levels of St.3 respiration (220 ± 15 pmol O2/sec*mg) compared to M (166 ± 13 pmol O2/sec*mg, P = 0.02) and O groups (170 ± 13 pmol O2/sec*mg, P = 0.014). There was no difference in St.3 respiration between M and O groups. Similar group differences were also observed for St. U and St. L respiration. The Y group exhibited a higher VO2 peak (46 ± 2.9 ml/min/Kg) compared to M (28 ± 1.8 ml/min/Kg, P<0.01) and O (21 ± 2.2 ml/min/Kg, P<0.01) groups. When the three groups were combined, St.3 respiration was positively correlated with VO2peak (R = 0.631, P<0.01), and negatively correlated with age (R = -0.324, P = 0.01), BMI (R =-0.371, P<0.01), fasting glucose (R = -0.252, P = 0.047), and fat mass (R = -0.516, P = <0.01).

Conclusions: Our data suggest that age related changes in body composition and aerobic fitness may be more important to mitochondrial dysfunction than chronological age per se.

References: 1. Petersen KF, Befroy D, Dufour S, Dziura J, Ariyan C, Rothman DL, DiPietro L, Cline GW, Shulman GI. (2003). Mitochondrial Dysfunction in the Elderly: Possible Role in Insulin Resistance. Science 300(5622):1140-2. 2. Conley KE, Jubrias SA, Esselman PC. Oxidative capacity and ageing in human muscle. (2000). J Physiol. 526 (Pt 1): 203-10. 3. Short KR, Bigelow ML, Kahl J, Singh R, Coenen-Schimke J, Raghavakaimal S, Nair KS. (2005). Decline in skeletal muscle mitochondrial function with aging in humans. Proc Natl Acad Sci U S A. 102(15):5618-23.

Keywords: Mitochondrial respiration, Permeabilized Fibers, Skeletal Muscle, Aging

O2k-Network Lab: US PA Pittsburgh Goodpaster B


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Organism: Human  Tissue;cell: Skeletal Muscle"Skeletal Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.  Preparation: Permeabilized Tissue"Permeabilized Tissue" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. 



HRR: Oxygraph-2k