Difference between revisions of "Dinitrophenole"
From Bioblast
| Line 17: | Line 17: | ||
:::# Weigh 3.7 mg of DNP (FW = 184.11). | :::# Weigh 3.7 mg of DNP (FW = 184.11). | ||
:::# Dissolve in 1.2 ml H<sub>2</sub>O. | :::# Dissolve in 1.2 ml H<sub>2</sub>O. | ||
:::# Neutralize with 1 | :::# Neutralize with 1 M KOH. | ||
:::# Adjust final volume to 2 ml and divide into 0.2 ml portions. | :::# Adjust final volume to 2 ml and divide into 0.2 ml portions. | ||
:::# Store at -20 °C. | :::# Store at -20 °C. | ||
Revision as of 11:58, 26 August 2016
- high-resolution terminology - matching measurements at high-resolution
Dinitrophenole
Description
2,4-dinitrophenol (C6H4N2O5; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.
Abbreviation: DNP
Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol
MitoPedia topics: Uncoupler
- Preparation of 10 mM DNP stock solution (dissolved in H2O):
- Caution: Toxic!
- Weigh 3.7 mg of DNP (FW = 184.11).
- Dissolve in 1.2 ml H2O.
- Neutralize with 1 M KOH.
- Adjust final volume to 2 ml and divide into 0.2 ml portions.
- Store at -20 °C.
