Difference between revisions of "Dikalov 2014 Antioxid Redox Signal"
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{{Publication | {{Publication | ||
|title=Dikalov SI, Harrison DG (2014) Methods for Detection of Mitochondrial and Cellular Reactive Oxygen Species. Antioxid Redox Signal 20:372β382. | |title=Dikalov SI, Harrison DG (2014) Methods for Detection of Mitochondrial and Cellular Reactive Oxygen Species. Antioxid Redox Signal 20:372β382. | ||
|info= | |info=[https://pubmed.ncbi.nlm.nih.gov/22978713/ PMID: 22978713 Open Access] | ||
|authors=Dikalov SI, Harrison DG | |authors=Dikalov SI, Harrison DG | ||
|year=2014 | |year=2014 |
Revision as of 16:14, 22 February 2021
Dikalov SI, Harrison DG (2014) Methods for Detection of Mitochondrial and Cellular Reactive Oxygen Species. Antioxid Redox Signal 20:372β382. |
Dikalov SI, Harrison DG (2014) Antioxid Redox Signal
Abstract: Significance: Mitochondrial and cellular reactive oxygen species (ROS) play important roles in both physiological and pathological processes. Different ROS, such as superoxide (O2β’β), hydrogen peroxide, and peroxynitrite (ONOOβ’β), stimulate distinct cell-signaling pathways and lead to diverse outcomes depending on their amount and subcellular localization. A variety of methods have been developed for ROS detection; however, many of these methods are not specific, do not allow subcellular localization, and can produce artifacts. In this review, we will critically analyze ROS detection and present advantages and the shortcomings of several available methods. Recent Advances: In the past decade, a number of new fluorescent probes, electron-spin resonance approaches, and immunoassays have been developed. These new state-of-the-art methods provide improved selectivity and subcellular resolution for ROS detection. Critical Issues: Although new methods for HPLC superoxide detection, application of fluorescent boronate-containing probes, use of cell-targeted hydroxylamine spin probes, and immunospin trapping have been available for several years, there has been lack of translation of these into biomedical research, limiting their widespread use. Future Directions: Additional studies to translate these new technologies from the test tube to physiological applications are needed and could lead to a wider application of these approaches to study mitochondrial and cellular ROS.
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