- high-resolution terminology - matching measurements at high-resolution
Cytochrome c
Description
Cytochrome c is a component of the electron transfer system (ETS) in mitochondria. It is a small heme protein loosely associated with the outer side of the inner mitochondrial membrane. The heme group of cytochrome c transfers electrons from Complex III to Complex IV. The release of cytochrome c into the cytoplasm is associated with apoptosis.
Abbreviation: c
Reference: MiPNet09.12; Gnaiger 2002 Biochem Soc Trans
MitoPedia topics: Substrate and metabolite
Application in HRR: storage and stock solution of c
c: Cytochrome c (from equine heart), Sigma C 7752, 50 mg, store at -20 °C; FW = 12500.
Preparation of 4 mM cytochrome c solution (dissolved in H2O):
- 1. Weigh 50 mg cytochrome c into a small glass beaker. Difficult to weigh, since the powder is electromagnetic.
- 2. Add 1 ml H2O; c dissolves easily.
- 3. Divide into 0.2 ml portions.
- 4. Store at -20 °C.
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Oxygraph-2k manual titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber)
- Final concwentration: 10 µM
Cytochrome c control: a test for outer mt-membrane integrity
- More details: >> Cytochrome c control factor
