Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Cytochrome c

From Bioblast


high-resolution terminology - matching measurements at high-resolution


Cytochrome c

Description

Cytochrome c is a component of the electron transport system (ETS) in mitochondria. It is a small heme protein loosely associated with the outside side of the inner mitochondrial membrane. The heme group of cytochrome c transfers electrons from complex III to the complex IV. The release of cytochrome c into the cytoplasm is associated with apoptosis. [1].

Abbreviation: c

Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans


MitoPedia topics: Substrate and metabolite 

Application in HRR

c: Cytochrome c (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500)

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 4 mM Cytochrome c solution (dissolved in H2O):

1) weigh 50 mg of Cytochrome c into a small glass beaker. Difficult to weigh, since the powder is electromagnetic.
2) add 1 ml H2O. Dissolves easily.
3) divide into 0.2 ml portions
4) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 5 µl using a 25 µl syringe
  • Final conc. in 2 ml O2k-chamber: 10 µM