Difference between revisions of "Cytochrome c"
From Bioblast
Line 18: | Line 18: | ||
'''Preparation of 4 mM Cytochrome ''c'' solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 4 mM Cytochrome ''c'' solution''' (dissolved in H<sub>2</sub>O): | ||
::1) weigh 50 mg of Cytochrome ''c'' into a small glass | ::1) weigh 50 mg of Cytochrome ''c'' into a small glass beaker. Difficult to weigh, since the powder appears to be electrostatic. | ||
::2) add 1 ml H<sub>2</sub>O. Dissolves easily. | ::2) add 1 ml H<sub>2</sub>O. Dissolves easily. | ||
::3) divide into 0.2 ml portions | ::3) divide into 0.2 ml portions |
Revision as of 10:52, 22 November 2010
Description
Abbreviation: c
Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans
Labels:
Application in HRR
c: Cytochrome c (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500)
Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening!
Preparation of 4 mM Cytochrome c solution (dissolved in H2O):
- 1) weigh 50 mg of Cytochrome c into a small glass beaker. Difficult to weigh, since the powder appears to be electrostatic.
- 2) add 1 ml H2O. Dissolves easily.
- 3) divide into 0.2 ml portions
- 4) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 10 µM