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Difference between revisions of "Cytochrome c"

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'''Preparation of 4 mM Cytochrome ''c'' solution''' (dissolved in H<sub>2</sub>O):
'''Preparation of 4 mM Cytochrome ''c'' solution''' (dissolved in H<sub>2</sub>O):


::1) weigh 50 mg of Cytochrome ''c'' into a small glass vial. Difficult to weigh, since the powder appears to be electrostatic.
::1) weigh 50 mg of Cytochrome ''c'' into a small glass beaker. Difficult to weigh, since the powder appears to be electrostatic.
::2) add 1 ml H<sub>2</sub>O. Dissolves easily.
::2) add 1 ml H<sub>2</sub>O. Dissolves easily.
::3) divide into 0.2 ml portions  
::3) divide into 0.2 ml portions  

Revision as of 10:52, 22 November 2010


high-resolution terminology - matching measurements at high-resolution


Cytochrome c

Description

Abbreviation: c

Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans


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Application in HRR

c: Cytochrome c (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500)

Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening!

Preparation of 4 mM Cytochrome c solution (dissolved in H2O):

1) weigh 50 mg of Cytochrome c into a small glass beaker. Difficult to weigh, since the powder appears to be electrostatic.
2) add 1 ml H2O. Dissolves easily.
3) divide into 0.2 ml portions
4) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 5 µl using a 25 µl syringe
  • Final conc. in 2 ml O2k-chamber: 10 µM