Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Cytochrome c"

From Bioblast
Line 13: Line 13:


''' ''c'': Cytochrome ''c'' ''' (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500)
''' ''c'': Cytochrome ''c'' ''' (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500)
Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening!
Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening!



Revision as of 10:37, 22 November 2010


high-resolution terminology - matching measurements at high-resolution


Cytochrome c

Description

Abbreviation: c

Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans


Labels:








Application in HRR

c: Cytochrome c (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500)

Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening!

Preparation of 4 mM Cytochrome c solution (dissolved in H2O):

1) weigh 50 mg of Cytochrome c into a small glass vial. Difficult to weigh, since the powder appears to be electrostatic.
2) add 1 ml H2O. Dissolves easily.
3) divide into 0.2 ml portions
4) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

  • Titration volume: 5 µl using a 25 µl syringe
  • Final conc. in 2 ml O2k-chamber: 10 µM