Difference between revisions of "Cytochrome c"
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''' ''c'': Cytochrome ''c'' ''' (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500) | ''' ''c'': Cytochrome ''c'' ''' (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500) | ||
Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening! | Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening! | ||
Revision as of 10:37, 22 November 2010
Description
Abbreviation: c
Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans
Labels:
Application in HRR
c: Cytochrome c (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500)
Chemicals stored in the fridge or the freezer should be adjusted to room temperature beforing opening!
Preparation of 4 mM Cytochrome c solution (dissolved in H2O):
- 1) weigh 50 mg of Cytochrome c into a small glass vial. Difficult to weigh, since the powder appears to be electrostatic.
- 2) add 1 ml H2O. Dissolves easily.
- 3) divide into 0.2 ml portions
- 4) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 10 µM