Difference between revisions of "Cytochrome c"
From Bioblast
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
''' | ''' ''c'': Cytochrome ''c'' ''' (L-Glutamic acid, sodium salt, C<sub>5</sub>H<sub>8</sub>NO<sub>4</sub>Na; contains 1 mol/mol H<sub>2</sub>O); Sigma G 1626 (R.T.): FW = 187.1 or 169.1 anhydrous) | ||
'''Preparation of 2 M L-Glutamic acid solution''' (dissolved in H<sub>2</sub>O): | '''Preparation of 2 M L-Glutamic acid solution''' (dissolved in H<sub>2</sub>O): | ||
Revision as of 11:20, 22 November 2010
- high-resolution terminology - matching measurements at high-resolution
Cytochrome c
Description
Abbreviation: c
Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans
Labels:
Application in HRR
c: Cytochrome c (L-Glutamic acid, sodium salt, C5H8NO4Na; contains 1 mol/mol H2O); Sigma G 1626 (R.T.): FW = 187.1 or 169.1 anhydrous)
Preparation of 2 M L-Glutamic acid solution (dissolved in H2O):
- 1) weigh 1.871 g of L-Glutamic acid and transfer to 5 mL volumetric glass flask
- 2) add 3 ml H2O (pH = 6)
- 3) neutralize with 5 N KOH (approx. 50 µl)
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 5 ml and divide into 0.5 ml portions.
- 6) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 10 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 10 mM
