Cytochrome c

high-resolution terminology - matching measurements at high-resolution

Cytochrome c

Description

Cytochrome c is a component of the electron transfer system (ETS) in mitochondria. It is a small heme protein loosely associated with the outer side of the inner mitochondrial membrane. The heme group of cytochrome c transfers electrons from Complex III to Complex IV. The release of cytochrome c into the cytoplasm is associated with apoptosis. [1].

Abbreviation: c

Reference: MiPNet09.12; Gnaiger_2002_BiochemSocTrans

MitoPedia topics: Substrate and metabolite 

Application in HRR

c: Cytochrome c (Sigma C 7752, 50 mg, store at -20 °C): FW = 12500)

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 4 mM Cytochrome c solution (dissolved in H₂O):

1) weigh 50 mg of Cytochrome c into a small glass beaker. Difficult to weigh, since the powder is electromagnetic.

2) add 1 ml H₂O. Dissolves easily.

3) divide into 0.2 ml portions

4) store frozen at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

• Titration volume: 5 µl using a 25 µl syringe
• Final conc. in 2 ml O2k-chamber: 10 µM