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Difference between revisions of "Cytochrome c"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=c
|abbr=c
|description='''Cytochrome ''c''''' is a component of the electron transfer system ([[ET-pathway]]) in mitochondria. It is a small heme protein loosely associated with the outer side of the inner mitochondrial membrane. The heme group of cytochrome ''c'' transfers electrons from [[Complex III]] to [[Complex IV]]. The release of '''cytochrome ''c''''' into the cytoplasm is associated with apoptosis.
|description='''Cytochrome ''c''''' is a component of the Electron transfer-pathway ([[ET-pathway]]) in mitochondria. It is a small heme protein loosely associated with the outer side of the inner mitochondrial membrane. The heme group of cytochrome ''c'' transfers electrons from [[Complex III]] to [[Complex IV]]. The release of '''cytochrome ''c''''' into the cytoplasm is associated with apoptosis.
|info=[[MiPNet09.12]]; [[Gnaiger 2002 Biochem Soc Trans]]
|info=[[MiPNet09.12]]; [[Gnaiger 2002 Biochem Soc Trans]]
|type=Substrate ETS
|type=Substrate ETS

Revision as of 13:56, 20 October 2017


high-resolution terminology - matching measurements at high-resolution


Cytochrome c

Description

Cytochrome c is a component of the Electron transfer-pathway (ET-pathway) in mitochondria. It is a small heme protein loosely associated with the outer side of the inner mitochondrial membrane. The heme group of cytochrome c transfers electrons from Complex III to Complex IV. The release of cytochrome c into the cytoplasm is associated with apoptosis.

Abbreviation: c

Reference: MiPNet09.12; Gnaiger 2002 Biochem Soc Trans



MitoPedia topics: Substrate and metabolite 

Application in HRFR: storage and stock solution of c

c: Cytochrome c (from equine heart), Sigma C 7752, 50 mg, store at -20 °C; FW = 12384 Da.


Preparation of 4 mM cytochrome c solution (dissolved in H2O):
  1. Weigh 50 mg cytochrome c into a small glass beaker. Difficult to weigh, since the powder is electromagnetic.
  2. Add 1 ml H2O; c dissolves easily.
  3. Divide into 0.2 ml portions.
  4. Store at -20 °C.
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
O2k manual titrations: MiPNet09.12 O2k-Titrations
  • Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
  • Final concentration: 10 µM.

Cytochrome c control: a test for outer mt-membrane integrity