Difference between revisions of "Cytochrome c"
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== Application in [[HRR]]: storage and stock solution of ''c'' == | == Application in [[HRR]]: storage and stock solution of ''c'' == | ||
''' ''c'': Cytochrome ''c'' ''' (from equine heart), Sigma C 7752, 50 mg, store at -20 °C; FW = 12500. | :::: '''''c'': Cytochrome ''c'' ''' (from equine heart), Sigma C 7752, 50 mg, store at -20 °C; FW = 12500. | ||
'''Preparation of 4 mM cytochrome ''c'' solution''' (dissolved in H<sub>2</sub>O): | ::: '''Preparation of 4 mM cytochrome ''c'' solution''' (dissolved in H<sub>2</sub>O): | ||
:: | ::::# Weigh 50 mg cytochrome ''c'' into a small glass beaker. Difficult to weigh, since the powder is electromagnetic. | ||
:: | ::::# Add 1 ml H<sub>2</sub>O; ''c'' dissolves easily. | ||
:: | ::::# Divide into 0.2 ml portions. | ||
:: | ::::# Store at -20 °C. | ||
'''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. | :::: '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening. | ||
'''O2k manual titrations:''' [[MiPNet09.12 O2k-Titrations]] | ::: '''O2k manual titrations:''' [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber) | ::::* Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber). | ||
::* Final | ::::* Final concentration: 10 µM. | ||
== Cytochrome ''c'' control: a test for outer mt-membrane integrity == | == Cytochrome ''c'' control: a test for outer mt-membrane integrity == | ||
* ''More details:'' | ::::* ''More details:'' » [[Cytochrome c control factor]] | ||
Revision as of 09:50, 2 June 2016
- high-resolution terminology - matching measurements at high-resolution
Cytochrome c
Description
Cytochrome c is a component of the electron transfer system (ETS) in mitochondria. It is a small heme protein loosely associated with the outer side of the inner mitochondrial membrane. The heme group of cytochrome c transfers electrons from Complex III to Complex IV. The release of cytochrome c into the cytoplasm is associated with apoptosis.
Abbreviation: c
Reference: MiPNet09.12; Gnaiger 2002 Biochem Soc Trans
MitoPedia topics: Substrate and metabolite
Application in HRR: storage and stock solution of c
- c: Cytochrome c (from equine heart), Sigma C 7752, 50 mg, store at -20 °C; FW = 12500.
- Preparation of 4 mM cytochrome c solution (dissolved in H2O):
- Weigh 50 mg cytochrome c into a small glass beaker. Difficult to weigh, since the powder is electromagnetic.
- Add 1 ml H2O; c dissolves easily.
- Divide into 0.2 ml portions.
- Store at -20 °C.
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- O2k manual titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 5 µl using a 25 µl syringe (2 ml O2k-chamber).
- Final concentration: 10 µM.
Cytochrome c control: a test for outer mt-membrane integrity
- More details: » Cytochrome c control factor
