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Difference between revisions of "Creatine"

From Bioblast
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== Application in [[HRR]]Β  ==
== Application in [[HRR]]Β  ==


'''Cr: Creatine ''' (Creatine monohydrate, C<sub>4</sub>H<sub>9</sub>N<sub>3</sub>O<sub>2</sub>*H<sub>2</sub>O ); Sigma 27900, 100 g, store at R.T.: FW = 149.15
'''Cr: Creatine ''' (creatine monohydrate, C<sub>4</sub>H<sub>9</sub>N<sub>3</sub>O<sub>2</sub>*H<sub>2</sub>O ); FW = 149.15; Sigma 27900, 100 g, store at R.T.




'''Preparation of MiR05Cr or MiR06Cr with 20 mM Creatine''': Β 
'''Preparation of MiR05Cr or MiR06Cr with 20 mM creatine''': Β 


<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span>
<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span>


::1) weigh 6 mg Creatine
::1) Weigh 6 mg creatine.
::2) dissolve in 2 ml [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]]
::2) Dissolve in 2 ml [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]].

Revision as of 16:56, 6 April 2015


high-resolution terminology - matching measurements at high-resolution


Creatine

Description

Creatine is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate (ATP).

Abbreviation: Cr

Reference: Saks 2000 Acta Physiol Scand



MitoPedia topics: Substrate and metabolite 

Application in HRR

Cr: Creatine (creatine monohydrate, C4H9N3O2*H2O ); FW = 149.15; Sigma 27900, 100 g, store at R.T.


Preparation of MiR05Cr or MiR06Cr with 20 mM creatine:

Caution: Prepare fresh everyday!

1) Weigh 6 mg creatine.
2) Dissolve in 2 ml MiR05 or MiR06 to obtain MiR05Cr or MiR06Cr.