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Difference between revisions of "Creatine"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=Cr
|abbr=Cr
|description='''Creatine'''Β  is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate ([[ATP]]). Β 
|description='''Creatine'''Β  is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate ([[ATP]]).
|info=[[Saks 2000 Acta Physiol Scand]]
|info=[[Saks 2000 Acta Physiol Scand]]
}}
}}
{{MitoPedia methods}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
|mitopedia topic=Substrate and metabolite
}}
}}
== Application in [[HRR]]Β  ==
== Application in [[HRR]]Β  ==


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'''Preparation of MiR07 with 20 mM Creatine''': Β 
'''Preparation of MiR05Cr or MiR06Cr with 20 mM Creatine''': Β 


<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!.</span>
<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!.</span>


::1) weigh 6 mg Creatine
::1) weigh 6 mg Creatine
::2) dissolve in 2 ml [[MiR06]]
::2) dissolve in 2 ml [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]]

Revision as of 07:58, 18 July 2012


high-resolution terminology - matching measurements at high-resolution


Creatine

Description

Creatine is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate (ATP).

Abbreviation: Cr

Reference: Saks 2000 Acta Physiol Scand



MitoPedia topics: Substrate and metabolite 

Application in HRR

Cr: Creatine (Creatine monohydrate, C4H9N3O2*H2O ); Sigma 27900, 100 g, store at R.T.: FW = 149.15


Preparation of MiR05Cr or MiR06Cr with 20 mM Creatine:

Caution: Prepare fresh everyday!.

1) weigh 6 mg Creatine
2) dissolve in 2 ml MiR05 or MiR06 to obtain MiR05Cr or MiR06Cr