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Difference between revisions of "Creatine"

From Bioblast
(replaced FW by M and added units g*mol-1)
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{{MitoPedia
{{MitoPedia
|abbr=Cr
|abbr=Cr
|description='''Creatine'''ย  is a nitrogenous organic acid that occurs naturally in vertebrates and helps to supply primarily muscle cells by increasing the formation of adenosine triphosphate (ATP). ย 
|description='''Creatine'''ย  is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate ([[ATP]]).
|info=[[Saks 2000 Acta Physiol Scand]]
|info=[[Saks 2000 Acta Physiol Scand]]
}}
}}
{{MitoPedia topics
{{MitoPedia methods}}
|mitopedia topic=Substrate and metabolite
__TOC__
}}
== Application in [[HRR]] ==


== Application in [[HRR]]ย  ==
::: '''Cr: Creatine ''' (creatine monohydrate, C<sub>4</sub>H<sub>9</sub>N<sub>3</sub>O<sub>2</sub>*H<sub>2</sub>O ); M = 149.15 gยทmol<sup>-1</sup>; Sigma 27900, 100 g, store at RT.


'''Cr: Creatine ''' (Creatine monohydrate, C<sub>4</sub>H<sub>9</sub>N<sub>3</sub>O<sub>2</sub>*H<sub>2</sub>O ); Sigma 27900, 100 g, store at R.T.: FW = 149.15


:::: '''Preparation of MiR05Cr or MiR06Cr with 20 mM creatine''':


'''Preparation of MiR07 with 20 mM Creatine''':
:::: <span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span>


<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!.</span>
::::# Weigh 6 mg creatine.
ย 
::::# Dissolve in 2 mL [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]].
::1) weigh 6 mg Creatine
{{MitoPedia topics
::2) dissolve in 2 ml [[MiR06]]
|mitopedia topic=Substrate and metabolite
}}

Latest revision as of 13:12, 12 February 2021


high-resolution terminology - matching measurements at high-resolution


Creatine

Description

Creatine is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate (ATP).

Abbreviation: Cr

Reference: Saks 2000 Acta Physiol Scand



Application in HRR

Cr: Creatine (creatine monohydrate, C4H9N3O2*H2O ); M = 149.15 gยทmol-1; Sigma 27900, 100 g, store at RT.


Preparation of MiR05Cr or MiR06Cr with 20 mM creatine:
Caution: Prepare fresh everyday!
  1. Weigh 6 mg creatine.
  2. Dissolve in 2 mL MiR05 or MiR06 to obtain MiR05Cr or MiR06Cr.

MitoPedia topics: Substrate and metabolite