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Difference between revisions of "Creatine"

From Bioblast
(replaced FW by M and added units g*mol-1)
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|mitopedia topic=Substrate and metabolite
== Application in [[HRR]] ==
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== Application in [[HRFR]] ==


'''Cr: Creatine ''' (creatine monohydrate, C<sub>4</sub>H<sub>9</sub>N<sub>3</sub>O<sub>2</sub>*H<sub>2</sub>O ); FW = 149.15; Sigma 27900, 100 g, store at R.T.
::: '''Cr: Creatine ''' (creatine monohydrate, C<sub>4</sub>H<sub>9</sub>N<sub>3</sub>O<sub>2</sub>*H<sub>2</sub>O ); M = 149.15 gยทmol<sup>-1</sup>; Sigma 27900, 100 g, store at RT.




'''Preparation of MiR05Cr or MiR06Cr with 20 mM creatine''': ย 
:::: '''Preparation of MiR05Cr or MiR06Cr with 20 mM creatine''': ย 


<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span>
:::: <span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span>


::1) Weigh 6 mg creatine.
::::# Weigh 6 mg creatine.
::2) Dissolve in 2 ml [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]].
::::# Dissolve in 2 mL [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]].
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
}}

Latest revision as of 13:12, 12 February 2021


high-resolution terminology - matching measurements at high-resolution


Creatine

Description

Creatine is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate (ATP).

Abbreviation: Cr

Reference: Saks 2000 Acta Physiol Scand



Application in HRR

Cr: Creatine (creatine monohydrate, C4H9N3O2*H2O ); M = 149.15 gยทmol-1; Sigma 27900, 100 g, store at RT.


Preparation of MiR05Cr or MiR06Cr with 20 mM creatine:
Caution: Prepare fresh everyday!
  1. Weigh 6 mg creatine.
  2. Dissolve in 2 mL MiR05 or MiR06 to obtain MiR05Cr or MiR06Cr.

MitoPedia topics: Substrate and metabolite