Difference between revisions of "Creatine"
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Cr: Creatine ''' (creatine monohydrate, C<sub>4</sub>H<sub>9</sub>N<sub>3</sub>O<sub>2</sub>*H<sub>2</sub>O ); FW = 149.15; Sigma 27900, 100 g, store at RT. | ::: '''Cr: Creatine ''' (creatine monohydrate, C<sub>4</sub>H<sub>9</sub>N<sub>3</sub>O<sub>2</sub>*H<sub>2</sub>O ); FW = 149.15; Sigma 27900, 100 g, store at RT. | ||
'''Preparation of MiR05Cr or MiR06Cr with 20 mM creatine''': | :::: '''Preparation of MiR05Cr or MiR06Cr with 20 mM creatine''': | ||
<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span> | <span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span> | ||
:: | ::::# Weigh 6 mg creatine. | ||
:: | ::::# Dissolve in 2 mL [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]]. | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Substrate and metabolite | |mitopedia topic=Substrate and metabolite | ||
}} | }} | ||
Revision as of 08:48, 11 September 2020
- high-resolution terminology - matching measurements at high-resolution
Creatine
Description
Creatine is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate (ATP).
Abbreviation: Cr
Reference: Saks 2000 Acta Physiol Scand
Application in HRR
- Cr: Creatine (creatine monohydrate, C4H9N3O2*H2O ); FW = 149.15; Sigma 27900, 100 g, store at RT.
- Preparation of MiR05Cr or MiR06Cr with 20 mM creatine:
Caution: Prepare fresh everyday!
MitoPedia topics: Substrate and metabolite
