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Difference between revisions of "Creatine"

From Bioblast
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{{MitoPedia methods}}
{{MitoPedia methods}}
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== Application in [[HRR]] ==
== Application in [[HRR]] ==


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::1) Weigh 6 mg creatine.
::1) Weigh 6 mg creatine.
::2) Dissolve in 2 mL [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]].
::2) Dissolve in 2 mL [[MiR05]] or [[MiR06]] to obtain [[MiR05Cr]] or [[MiR06Cr]].
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
}}

Revision as of 17:19, 10 September 2020


high-resolution terminology - matching measurements at high-resolution


Creatine

Description

Creatine is a nitrogenous organic acid that occurs naturally in vertebrates and helps primarily muscle cells to supply energy by increasing the formation of adenosine triphosphate (ATP).

Abbreviation: Cr

Reference: Saks 2000 Acta Physiol Scand



Application in HRR

Cr: Creatine (creatine monohydrate, C4H9N3O2*H2O ); FW = 149.15; Sigma 27900, 100 g, store at RT.


Preparation of MiR05Cr or MiR06Cr with 20 mM creatine:

Caution: Prepare fresh everyday!

1) Weigh 6 mg creatine.
2) Dissolve in 2 mL MiR05 or MiR06 to obtain MiR05Cr or MiR06Cr.

MitoPedia topics: Substrate and metabolite